to examine the efficacy of the fungus Trichoderma harzianum, culture age, inoculum type, application time, fungal extract, compost and bacterial strain on Striga hermonthica germination and sorghum infestation. The highest significant (P≤0.05) inhibition on S. hermonthica germination was obtained at 10 days by T. harzianum culture as compared to both controls. Application of all T. harzianum aqueous and ethyl acetate extracts concentrations significantly (P≤0.05) reduced S. hermonthica seed germination as compared to the corresponding control. T. harzianum inoculum extracted by ethyl acetate reduced germination by 97%. T. harzianum aqueous 100% induced germination during conditioning by 64 % in response to GR24 (0.1ppm). All types of T. harzianum inoculum (Autoclaved, culture and filtrate) significantly (P≤0.05) reduced germination, with application of T. harzianum culture filtrate gave the highest reduction on germination as compared to control and other inoculums. Application of the 3 inoculums at 2 hours reduced germination percentage more than at 4 hours. Filtrate and culture inoculums at 2 hours reduced germination by 79 and 68%, respectively. The combination of compost 100%+ T. harzianum + BMP+Flavobacterium reduced germination by 68%. The greenhouse results showed that the combination of compost plus BMP+ Flavobacterium gave lowest number of S. hermonthica emergence and the highest sorghum plant height. The combinations of compost with T. harzianum and with BMP+ Flavobacterium significantly reduced S. hermonthica dry weight, increased sorghum shoot and root dry weight insignificantly as compared to the control.
A series of laboratory and field experiments were undertaken at the laboratories and experimental farm of Environment, Natural Resources and Desertification Research Institute, NCR and Shendi Research Station experimental farm, ARC, Sudan at season 2015-2016, to evaluate efficacy of Trichoderma harzianum, Bacillus megatherium var. Phosphaticum (BMP), Rhizobium leguminosarum (TAL1399) and the herbicide imazethapyr (pursuit) against Orobanche crenata infesting faba bean. Treatments were laid out in a Complete Randomized Design (CRD) in laboratory experiment and in a Randomized Complete Block Design (RCBD) in the field experiments with four replicates. Results of laboratory experiments showed that T. harzianum and Imazethapyr each alone or in combination significantly reduced O. crenata germination. Field results revealed that, application of T. harzianum, Imazethapyr and Imazethapyr + BMP+TAL1399 significantly delayed the days of O. crenata emergence in Shendi and Soba sites. T. harzianum alone or in combination with bacteria or imazethapyr significantly reduced number of O. crenata emergence and increased faba bean plant height as compared to the corresponding control in Shendi and Soba sites. T. harzanium alone or in combination with T. harzanium + BMP+TAL1399 significantly increased faba bean biomass, pod/plant and grain yield and 100 seed weight insignificantly as compared to the infested control in Shendi and Soba sites.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.