Objective: To develop and validate a new simple, accurate, precise and sensitive high performance liquid chromatographic method (HPLC) method for simultaneous estimation of ubidecarenone and vitamin E acetate in capsule dosage form as per international conference on harmonization (ICH) guidelines. Methods: The chromatographic separation of drugs were achieved using hypersil C8 column (250 mm x 4.6 mm, 5µ) in isocratic elution mode with a mobile phase of methanol: ethanol: n-hexane (80:10:10 v/v/v) at a flow rate of 1 ml/min with ultra-violet (UV) detection at 210 nm. Results: The optimized method produced sharp peaks with good resolution, minimum tailing factor and satisfactory retention time were found to be 5.745 min and 12.565 min for vitamin E acetate and ubidecarenone respectively. The method was linear in the range of 60-180 µg/ml for ubidecarenone and 20-60 µg/ml for vitamin E acetate with a correlation coefficient of 0.999 and 0.9993 respectively. Mean recoveries observed for ubidecarenone and vitamin E acetate were 99.85% and 99.73% respectively. The percentage relative standard deviation (% RSD) of peak area for system precision, method precision, and intermediate precision were found to be less than 0.37%. The lower degree of % RSD obtained has proved that the method was precise and robust. Conclusion: A new simple HPLC method was developed and validated as per ICH guidelines for the simultaneous estimation of ubidecarenone and vitamin E acetate and the method can be effectively applied for the routine analysis of active pharmaceutical ingredient (API) and formulations.

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Some Bis indole substituted coumarin derivatives prepared in 3 steps. The final compound III is react with different substituted indole compounds and aceto nitrile at room temperature for 30 minutes the compounds S1-S6 is obtained. The present investigation deals with the synthesized compounds having anti hyperlipidemic activity. Table 1 and 2 show the levels of Cholesterol, Triglycerides, HDL, LDL and VLDL of control and experiment rats respectively. Serum of hyperlipidemic rats showed significantly increased levels of Cholesterol, Triglycerides, LDL -C and low HDL -C, when compared with normal rats. In rats treated with various synthetic drugs and Atorvastatin there was significant decrease in the content cholesterol, TGs, LDL -C, and VLDL and increases HDL -C, when compared with cholesterol control rats.

:There are many kinds of literatures were available for thiocolchicoside and lornoxicam in tablets and capsules formulation by different analytical methods such as fluorimetry, RP-HPLC, and UV, etc. Hence, this article brings a cumulative and communicative presentation of available analytical research works and was presented as a review of analytical methods for the determination of lornoxicam and thiocolchicoside in bulk and its dosage form. In this article, we summarized the available analytical methods reported so far in and around the world. The methods include UV-VIS spectrometry, HPLC, HPTLC, Stability-indicating methods with their utility for determination of thiocolchicoside and lornoxicam in biological matrices, bulk material, and different pharmaceutical formulations.