The aim of the present study was to investigate biological properties of the novel nitrobenzoxadiazole derivative 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan. Materials and Methods:We used a bioluminescent test based on a set of lux-biosensors, which are genetically modified E.coli strains able to react on different types of factors that can induce an SOS-response with light emission. The spontaneous and induced mutation frequencies of antibiotic resistance in E. coli were determined by methods of classical genetics of microorganisms.Results: 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan demonstrated inhibition of SOS-response in a biosensor model system and significantly reduced the frequency of spontaneous mutations and mutations induced by ciprofloxacin of antibiotic resistance.Conclusion: Based on our data, we can recommend using compound 1 as a starting point for the development of drugs that block mutagenesis associated with the emergence of antibiotic-resistant bacteria. (Int J Biomed. 2016;6(3):228-232.).

Objective: to study the effect of the total expression of vascular endothelial growth factor A (VEGF A), tissue growth factor β1 (TGF-β1) and monocytic chemoattractant factor 1 (MCP-1) on the course of glomerulonephritis.Material and methods: the study was a prospective (12-month) open comparative clinical and morphological study with the inclusion of 75 patients aged 18 to 75 years (males - 52, females - 23) with glomerulonephritis in the acute stage. All patients were initially examined, including assessment of clinical, laboratory and instrumental parameters, including puncture nephrobiopsy. In addition to the standard, sections of biopsies were stained with labeled antibodies to VEGF A, TGF-β1 and MCP-1 to assess their expression in kidney tissue. After 12 months, the development of remission of glomerulonephritis, parameters of the general analysis of urine, creatinine, urea, uric acid of blood, calculation of GFR were evaluated.Results: it was found that the severity of the total expression of MCP-1+VEGF-A+TGFß1 is observed to a greater extent in hematuric forms of glomerulonephritis, occurring without changes in the level of blood albumin. The same follows from the results demonstrating the relationship of the total expression of MCP-1+VEGF-A+TGFß1 with morphological manifestations more characteristic of nephritic forms of glomerulonephritis (IgA deposits, mesangial hypercellularity). The increased expression of the studied factors has shown its influence on the development of fibrosis and thickening of the walls of renal vessels, glomerular fibrosis and interstitium, reflecting the whole complex of reparative remodeling of renal tissue in the inflammatory process. The effect of the total expression of MCP-1+VEGF-A+TGFß1 on the further course of glomerulonephritis, accompanied by the progression of the disease in the form of a decrease in GFR after 12 months of observation of patients, was revealed.Conclusions: an increase in the total expression of MCP-1+VEGF-A+TGFß1 in hematuric forms of glomerulonephritis indicates the role of these factors in the development of the inflammatory process to a greater extent in these forms of the disease. It was found that an increase in the severity of the total expression of MCP-1+VEGF-A+TGFß1 is associated with manifestations of renal remodeling in glomerulonephritis. It has been shown that an increase in the severity of the total expression of MCP-1+VEGF-A+TGFß1 is accompanied by an increase in the likelihood of a rapid decrease in renal function in glomerulonephritis at 12-month follow-up.

No abstract

Currently, there is a high scientific interest in studying the features of the structure and functions of the tubules of the kidneys. The relevance of the topic is due to the potential possibility of identifying various markers of tubular dysfunction and using them for early diagnosis of not only tubulopathies, but also glomerular disorders. In clinical practice, markers of tubular dysfunction are used insufficiently. The article presents information about the anatomical and functional features of the proximal and distal parts of the tubular apparatus, outlines highly organized mechanisms of intermolecular interaction, presents the main biologically active substances, the change in the concentration of which is a consequence of damage to the tubules. The presented manuscript is the product of a deep analysis and systematization of the available data in Russian and foreign information and analytical portals.