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Present investigation was undertaken to detect and characterize the PPR virus from different clinical tissue samples of 14 sheep and 17 goats with respiratory disease from Maharashtra, India. All animals were tested by Sandwich ELISA, of which 70.96% were found positive carrying high PPR virus inwhich 12 were sheep and 10 were goats respectively. For confirmation of PPR, molecular detection was performed with RT-PCR using F gene and N gene specific primers. Intestine samples accounted for highest percent positivity (75%) followed by blood (66.66%) and lymph node (62.5%) for presence of virus. Unusually higher positivity was observed in heart, liver and Kidney (60%, each) than normal predilection sites such as lungs (57.84%) and spleen (50%). While nasal swabs and blood were individually processed with F gene and N gene specific PCR, the triturated organs were pooled for processing into ‘Sample A’ comprised of heart, kidney, liver and intestine combined together and ‘Sample B’ comprising of lung, spleen and lymph nodes combined for the molecular detection of PPR yielding the products each of 372bp and 463bp sizes, respectively. Out of total 40 samples tested, 09/12 each from both sample A and B, while 02/10 nasal swabs resulted positive, respectively and all 06 blood samples remained negative. (F as well as N gene PCR methods were found best suitable for detection of PPR virus from tissue samples of small ruminants).

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| The presence of Salmonella spp in collected faecal samples was assessed by performing the pre-enrichment and enrichment culture, followed by PCR assay. The primer was selected from the invA gene, specific for the detection of Salmonella spp. It was observed that 28% (10/35) of Salmonella were isolated by the conventional method. All Salmonella isolates thus obtained were subjected to PCR for the invA gene and all were found positive. In order to provide a more accurate profile of the prevalence of Salmonella spp in faecal samples, it was pertinent to use invA gene specific PCR method that could be considered as rapid technique for identification of Salmonella spp.