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Eur J Oral Sci /998. 106 (supp/1 ). 384 387 P,.inted in UK. All rights resen ed Immunocytochemical detection of apoptosis in human odontoblasts Franquin J-C. Remusal M, Abou Hashieh I, Dejou J: lmmunocylochemical detection of apoptosis in human odontoblasts. Eur J Oral Sci 1998: 106 (suppl 1): 384 387. Eur J Oral Sci , 1998Pulpal chamber ize decrea es on ageing due to primary and secondary dentin deposition . This work was designed to find out the con equences of thi pulp chamber reduction on odontobla t number and distribution . Twenty-one healthy human premolars were equally divided into three groups from II-, 12.5-and 14-yr-old adolescents, respectively). The external and the internal perimeters of dentin were recorded on vestibulo-lingual sections, from buccal to lingual cemento-enamel junction using an image analysis system . Nuclei of the odontoblasts were recorded on 12 automatically selected fields. On nine erupted premolars (3 teeth from each II-, 12.5-and 14-yr-old patients) , apoptosis wa detected by confocal micro copy u ing a modification of the original TUNEL method . Apoptotic cell were labeled in central pulp fibroblasts , perivascular endothelial cells, and in odontoblasts. When the pulp volume decreases due to primary dentin production, the decrease of the surface available for odontobla t is compen a ted for by a multilayer distribution of cells. Secondary dentin deposition, associated with odontoblasts reorganization in a single layer, results in a hyperbolic decrease of the odontoblasts number. This decrease seems to result from a programmed cell death, which eliminates half of the odontoblasts over a 4-yr period .