Diabetic diseases often witnessing complication with dyslipidemia that also easily experiencing status of oxidative stress. This study aimed to determine the potential of Nigella sativa L. seed extract in reducing lipoprotein serum and liver oxidative stress in diabetic dyslipidemia. Diabetic dyslipidemia rats were carried out by giving a high feed diet (HFD) for 14 weeks and injecting streptozotocin (STZ) at dose 30 mg/kgbw intraperitoneally once a week at the beginning of 9th and 10th week. The treatments of Nigella sativa L. seed extracts were given between 11th -14th week. The ANOVA test showed that the Nigella sativa L. extract and metformin had a significant effect on LDL, HDL, and SOD levels. In addition, the DMRT test indicated that administration of the extract at the dose of 24-48 mg/kgbw was the most effective treatment in reducing LDL level, as well as in increasing HDL and SOD levels. The Kruskal Wallis test showed that the administration of Nigella sativa L. extract has effected on MDA level; furthermore, Mann Whitney test revealed that giving the extract at all doses were effective in reducing MDA. This effect met the standard of metformin drug until the animals got healthy again. It can be concluded that the antioxidant and hypolipidemic effects of Nigella sativa L. extract are potential in reducing the risk of cardiovascular disease in patients with diabetic hyperlipidemia.