Acne is the most common cause of patients' treatment of dermatologists. It is known that in the development of the disease, along with the dysfunction of the sebaceous glands of the skin, plays a skin microflora, namely, staphylococci and propionibacteria. In this regard, in the schemes of complex treatment of acne, local medicinal forms of antibiotics are widely used. The aim of the work was to investigate the composition of the microflora contained in the contents of pustules with acne and to study its sensitivity to antibiotics in order to assess the effectiveness of modern methods of treating patients with acne. The contents of the efflorescences of 55 patients with acne have been studied. Isolated and identified 49 strains of bacteria. Their sensitivity to antibiotics of various chemical groups by the disco-diffusion method was investigated. The level of sensitivity to antibiotics was determined by the percentage of sensitive strains among the isolates. Quantitative determination of sensitivity to antibiotics was carried out by serial dilution of preparations in a liquid nutrient medium. It was not possible to isolate bacteria from the contents of pustules in 18.2% of patients. In 77.2% of the examined, microorganisms of the genus Staphylococcus were isolated. In 28.9% of the patients, the propionibacterium was excreted in the contents of vesicular elements in monoculture and in association with staphylococci. The sensitivity of the isolates of staphylococci to beta-lactam antibiotics varied over a wide range and oxacillin was low (34.3%) and high to cefazolin (97.1%). Low sensitivity was isolated strains of staphylococci to antibiotics most commonly used in dermatological practice in integrated circuits acne treatment, namely: clindamycin — 45.7%, chloramphenicol — 34.3%, erythromycin — 17.1%. Similar characteristics of sensitivity to antibiotics are established for isolated strains of propionibacteria. The current state of the antibiotic resistance of microorganisms involved in the development of acne requires the correction of local antibiotic regimens for this disease. Appropriate adjustments should be made based on the results of a study of the sensitivity of the isolated microflora to antiseptics, to which resistance in microorganisms is formed slowly.