Surface proteins of Staphylococcus aureus play vital roles in bacterial physiology and pathogenesis. Recent work suggests that surface proteins are spatially regulated by a YSIRK/GXXS signal peptide that promotes cross-wall targeting at the mid-cell, though the mechanisms remain unclear. We previously showed that protein A (SpA), a YSIRK/GXXS protein and key staphylococcal virulence factor, mis-localizes in a ltaS mutant deficient in lipoteichoic acid (LTA) production. Here, we identified that SpA contains another cross-wall targeting signal, the LysM domain, which, in addition to Strains or plasmids Description Reference or source E. coli DC10B Cloning strain Monk et al. (2012) S. aureus SEJ1 RN4220∆spa, WY112 Gründling and Schneewind (2007a) SEJ1iltaS RN4220∆spa P spac -ltaS, Ery R , IPTG inducible, WY443 Gründling and Schneewind (2007b) ANG1786 SEJ1∆ltaS suppressor 4S5, WY449 Corrigan et al. (2011) WY109 RN4220 sbi::φΝΣ, Ery R Yu et al. (2018) WY110 RN4220∆spa∆sbi::φΝΣ, Ery R Yu et al. (2018) WY478 SEJ1 pCLitet-spa, Chl R , ATc-inducible This study WY745 SEJ1 pCLitet-spa ΔlysM , Chl R , ATc-inducible This study WY480 SEJ1 pCLitet, Chl R , ATc-inducible This study WY1026 WY110 pCLitet-spa, Chl R , ATc-inducible This study WY946 WY110 pCLitet-spa ΔlysM , Chl R , ATc-inducible This study WY1024 WY110 pCLitet, Chl R , ATc-inducible This study WY747 SEJ1iltaS pCLitet-spa, Chl R , Ery R , ATc and IPTG inducible This study WY748 SEJ1iltaS pCLitet-spa ΔlysM , Chl R , Ery R , ATc and IPTG inducible This study WY794 SEJ1iltaS pCLitet, Chl R , Ery R , ATc and IPTG inducible This study WY477 ANG1786 pCLitet-spa, Chl R , ATc-inducible This study WY777 ANG1786 pCLitet-spa ΔlysM , Chl R , ATc-inducible This study WY479 ANG1786 pCLitet, Chl R , ATc-inducible This study WY541 ANG1786 pCLitet-spa, pJK4, Chl R , Kan R , ATc and IPTG inducible This study WY543 ANG1786 pCLitet-spa, pJK4-ltaS, Chl R , Kan R , ATc and IPTG inducible This study WY545 ANG1786 pCLitet-spa, pJK4-ltaS T300A , Chl R , Kan R , ATc and IPTG inducible This study
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