Objective To determine if the GSTM1 null genotype is a risk factor for increased inflammatory response to inhaled endotoxin. Methods 35 volunteers who had undergone inhalation challenge with a 20 000 endotoxin unit dose of Clinical Center Reference Endotoxin (CCRE) were genotyped for the GSTM1 null polymorphism. Parameters of airway and systemic inflammation observed before and after challenge were compared in GSTM1 null (n=17) and GSTM1 (n=18) sufficient volunteers. Results GSTM1 null volunteers had significantly increased circulating white blood cells (WBCs), polymorphonuclear neutrophils (PMNs), platelets and sputum PMNs (% sputum PMNs and PMNs/mg sputum) after CCRE challenge. GSTM1 sufficient volunteers had significant, but lower increases in circulating WBCs, PMNs and % sputum PMNs, and no increase in platelets or PMNs/mg sputum. Linear regression analysis adjusted for baseline values of the entire cohort revealed that the GSTM1 null genotype significantly increased circulating WBCs, platelets and % sputum PMNs after challenge Conclusion These data support the hypothesis that the GSTM1 null genotype is a risk factor for increased acute respiratory and systemic inflammatory response to inhaled CCRE. These data are consistent with other observations that the GSTM1 null genotype is associated with increased respiratory, systemic and cardiovascular effects linked to ambient air particulate matter exposure and indicate that the GSTM1 null genotype should be considered a risk factor for adverse health effects associated with exposure to environmental endotoxin.

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ARGENTINA The m a j o r i t y o f t h e p a t i e n t s s u r g i c a l l y t r e a t e d f o r b i l a t e r a lcryptorchidism b e f o r e puberty, a r e s t e r i l e . It seems t h a t t h i s could be due more t o a primary t e s t i c u l a r damage t h a n t o t h e age when s u r g i c a l c o r r e c t i o n was performed. Ten boys with monorchid (hi), 1 0 u n i l a t e r a l (LCQ) and 31 b i l a t e r a l c r y p t o r c h i d (DCQ) t e s t e s were s t u d i e d t o e v a l u a t e t e s t i c u l a r f u n c t i o n b e f o r e adulthood i n an i n t e n t t o p r e d i c t f u t u r e f e r t i l i t y . LI; and FSH l e v e l s were measured by R I A before and a f t e r Gn-RII (25 ug) administrat i o n . No d i f f e r e n c e between hi, LCQ and BCQ was found a t prepub e r t a l age. P a t i e n t s with BCQ a t p u b e r t a l age presented t v o dif f e r e n t responses: Group I (n=4) Ill ( ; -3C. S) b a s a l (b) 2 R i maximal response (mr) 1 4 2 3 0 ; FSH ( b ) 86_+22, (mr) 1 6 2 4 9 . Group I1 (n=6) LH (b) 71519, (mr) 38949; FSH (b) 333f40, (mr) 9172184, being s i g n i f i c a n t l y d i f f e r e n t LH response (p 0.01) and b and mr.FSH ( p 0.0025 and p 0.01 r e s p e c t i v e l y ) . I n 4 p a t i e n t s from t h e s e two groups t e s t i c u l a r byopses were obtained d u r i n g o r c h i d g pexy and demonstrated complete spermatogenesis i n p a t i e n t s o f Group I and s e v e r e t e s t i c u l a r damage i n Group 11. I n conclusion, Gn-RII s t i m u l a t i o n i n puberty g i v e s i n t e r e s t i n g d a t a r e g a r d i n g f k p~~.~~f;e?rL-;t-~ga~r~"g~fs i n b i l a t e r a l cryptorchid p a t i e n t s t r e a -QUANTITATION OF GERhiINAL EPITHELILhi I N PREPUBERTAL H i 14 MAN CRYPTORCHIDISM. GOTTLIEB S i l v i a , CHEhBS H., VILAR O., BERGADA C. CEDIE, Hospital de Nifios. Buenos Aires. ARGENTINA. There i s no u n i v e r s a l agreement about t h e t i m e o f treatment of cryptorchidism i n o r d e r t o p r e w n t s t e r i l i t y a f t e r puberty. Teg t i c u l a r biopsies were embedded i n Epon-Araldita and s e c t i o n o f 1~ were examined. S e r t o l i and germ c e l l s were i d e n t i f i e d . 17 t e s t i c u l a r biopsies from c r y p t o r c h i d t e s t e s (CQ) and 5 c o n t r a l a t e r a 1 s c r o t a 1 t e s t e s (EX) i n p a t i e n t s from 1 t o 10 y e a r s were s t u d i e d . S e r t o l i (s) and germ c e l l (G) q u a n t i t a t i o n was c a r r i e d o u t . The values obtained were ( ; %FN) S: 1535i82 (CQ) and 1835 ( SC) and G: 104215 (CQ) and 213-' 79 (SC). Evaluation o f t h e r e l a t i o n s h i p between number of germ c e l l s p e r l ( W S e r t o 1 i c e l l sr e v e a l s 2 d i f f e r e n t groups: Group A (n=12): 49% and Group B (n-5): 128219. In conclusion: 1 ) t h e number o f S e r t o l i c e l l s is p r a c t i c a l l y c o n s t a n t , independently o f t h e t e s t i c u l a r p o s i t i o n and t r e a t m e n t , 2 ) t h e l a c k o f development o f t h e germinal e p i t k l i u m o f t h e c r ...

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