Introduction:Cisplatin is an effective chemotherapeutic agent that is active against malignant salivary gland tumors. However, cisplatin causes various concomitant adverse effects, such as mucostis, hyposalivation, and cytotoxicity which are associated with high morbidity and mortality. Annona muricata a strong antioxidant was considered effective against inflammatory, oxidative stress and free radical-induced damage.Objective: Investigation of the possible ameliorating effects of Annona muricata against cisplatin induced injury in parotid gland of albino rats. Material and methods:Thirty adult male Sprague dawley rats weighing from 200 -250 gm and 2.5-3 months of age were divided into 3 equal groups. Group I: received 6 mg /kg body wt. of saline by i.p. injection. Group II: received 6 mg /kg body wt. of cisplatin once by i.p. injection. Group III: received 6 mg /kg body wt. of cisplatin once by i.p. injection, then after 3 days animals start receiving 200 mg / kg body wt. of annona orally for 40 days. Five animals of each group were sacrificed with over dose anesthesia after 20 and 40 days from the beginning of treatment with annona. Parotid glands were excised and prepared for routine histological examination using heamatoxylin and eosin stain, Caspase 3 immunohistochemistry, enzyme linked immunosorbent assay of catalase enzyme activity and malondialdehyde.Results: Administration of Annona muricata ameliorate the histological picture and tissue architecture of the parotid glands, decrease the apoptotic effect and the lipid peroxidation induced by cisplatin and increase the level of catalase enzyme. Conclusion: Annona muricata extract has shown considerable antioxidant ameliorating effect against the undesirable effect of cisplatin, which could be considered for further investigation towards therapeutic application.

No abstract

Objectives: This study was conducted to evaluate the effect of two single centrifugation speeds used in platelet rich plasma (PRP) preparation on the expression of osteoclastogenic markers RANK, RANKL and the osteogenic marker Alkaline phosphatase (ALP) in experimentally induced periodontitis in a rat model. Material and Methods:Thirty four adult male Wistar rats, weighing 150-200 g were selected. Four rats for PRP preparation. Ligatures were placed subgingivally around the lower incisors of thirty rats in figure 8 manner. Ligature control was performed daily then after 14 days ligature removal was done and the rats were subsequently divided into 3 equal groups (n=10); group I: control non treated, group II: injected with 0.2ml PRP prepared with 800 rpm, group III: injected with 0.2ml PRP prepared by 3000 rpm. Five rats of each group were sacrificed after 3 and 6 weeks, the specimens were processed, then evaluated using haematoxylin and eosin, RANK, RANKL and ALP. The results were subjected to digital morphometric assessment followed by statistical analysis.Results: Both PRP treated groups showed better alveolar bone regeneration compared to control non treated group especially at the 6 th week. Group II had better regenerative capacity compared to group I and III as it showed significant increase in the amount of ALP and significant decrease in the RANK and RANKL osteoclastogenic markers. Conclusion:Local injection of PRP with both tested centrifugation speeds enhanced the regenerative capacity of bone in experimentally induced periodontitis. However, the lower centrifugation speed (800 rpm) have better regenerative effect.

No abstract