The aim of this study was to evaluate physicochemical properties and bioactive potential of Sealer Plus, in comparison with MTA Fillapex, Sealapex and AH Plus. Setting time, flow, and radiopacity were evaluated based on ISO 6876 Standard. Flow was also assessed in area (mm²). The solubility and volumetric change of the sealers were evaluated after 7 and 30 days of immersion in distilled water. Solubility was evaluated by the difference in mass of materials before and after immersion. Volumetric change was evaluated by using microcomputed tomography (micro-CT). The bioactive potential was observed by Scanning Electron Microscopy (SEM) after immersion in PBS. Data were compared using ANOVA and Tukey tests (α=0.05). Sealer Plus presented the shortest setting time (196 min.) and Sealapex the longest (912 min.) (p<0.05). AH Plus showed the highest radiopacity (9.5 mm Al) and MTA Fillapex the lowest (2.7 mm Al) (p<0.05). All the sealers presented flow in accordance with ISO 6876/2012 (>17 mm). Sealer Plus showed low solubility and volumetric change (<1%), and MTA Fillapex showed the highest solubility (>25%), and volumetric change (>4%) after all time intervals (p<0.05). MTA Fillapex was the only sealer that showed bioactive potential. In conclusion, Sealer Plus presented proper physicochemical properties. However, this sealer did not present a bioactive potential.