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Recebido em 5/10/07; aceito em 11/7/08; publicado na web em 2/12/08 IMMOBILIZATION OF THE ENZYMES ON CHROMATOGRAPHIC SUPPORTS: A TOOL TO RESEARCH OF INHIBITOR COMPOUNDS. The development and characterization of bioreactors or IMER (immobilized enzyme reactors) as research tools are important in the scope of medicinal chemistry and constitute an alternative for the rational development of drugs. This approach does not require highly purified enzymes or a great amount of protein, but increase the enzymatic stability against heat, organic solvents and pH, without too much loss of catalyst activity. Immobilized enzyme reactors (IMER) can be used for the accomplishment of high efficiency screening on-line and, thus inhibitors can be quickly identified. Here, we emphasize the development of IMER by use of different methods of immobilization and chromatographic supports. Their applications, in different areas of research, are also fully discussed.

This review presents an update on the use of restricted-access materials (RAMs) for direct injection of biological samples. The fundamental improvements in the preparation of tailored RAMs and the diversity of applications with these phases are presented. Insights into diminishing the matrix effect by the use of RAM supports in methods by LC-MS and into the low number of methods for enantiomeric separations by direct injections of biological samples are addressed. The diversity of systems that incorporate RAMs for selective sample clean-up or fractionation in proteome and peptidome analysis is also covered.