The detected proteins may be capable of predicting response to DOX therapy. This is a key tool in the treatment of breast cancer, and the combination with vit C seems to be of particular interest due to the fact that it can potentiate anti-proliferative effect of DOX.

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Open Life Sci. 2017; 12: 248-265 revealed information about potential risk factors associated with the development of caries-susceptibility and provides a better understanding of tooth protection mechanisms.Keywords: saliva; proteins; caries-susceptible; cariesfree; proteomic analysis; bioinformatics IntroductionDental caries, resulting in demineralization of the tooth structure, is ranked among the most prevalent chronic diseases of people worldwide [1][2][3]. Although it is not a lifethreatening disorder, it still represents a serious health issue with a significant effect on general health and quality of life [4,5].A complex set of interactions between acid producing bacteria and fermentable carbohydrates contribute to caries-risk [6,7]. Other factors associated with caries are saliva properties [8], genetic predispositions [9,10], age and immunological status, and behavioral factors like nutrition level and hygiene habits [2,11].The diagnostic assessment using the molecular analysis (e.g., to find protein markers) related to formation of tooth decay at early stages may help to identify risk factors and help with dental screening and personalized dental treatment.Recently, intensive investigation of protein functions in saliva as possible indicators for predicting caries-risk has begun using the state-of-the-art methodologies such as metabolomics, genomics, proteomics and bioinformatics [12].Vitorino et al. [13] published an early proteomic analysis evaluating the proteome of human saliva and the level of protein expression and adsorption on the human dental enamel surface. The authors compared the whole saliva proteome of male individuals having no dental caries to those afflicted with dental caries. Both saliva incubated with hydroxyapatite as well as in vivo extracts from the Abstract: Background: Dental caries disease is a dynamic process with a multi-factorial etiology. It is manifested by demineralization of enamel followed by damage spreading into the tooth inner structure. Successful early diagnosis could identify caries-risk and improve dental screening, providing a baseline for evaluating personalized dental treatment and prevention strategies. Methodology: Salivary proteome of the whole unstimulated saliva (WUS) samples was assessed in caries-free and caries-susceptible individuals of older adolescent age with permanent dentition using a nano-HPLC and MALDI-TOF/TOF mass spectrometry. Results: 554 proteins in the caries-free and 695 proteins in the caries-susceptible group were identified. Assessment using bioinformatics tools and Gene Ontology (GO) term enrichment analysis revealed qualitative differences between these two proteomes. Members of the cariessusceptible group exhibited a branch of cytokine binding gene products responsible for the regulation of immune and inflammatory responses to infections. Inspection of molecular functions and biological processes of caries-susceptible saliva samples revealed significant categories predominantly related to the activity of proteolytic peptidases, ...

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Despite the fact that proteomic analysis is becoming widely used in various medical branches its use in psychiatry is still very limited. Majority of psychiatric proteomic research is still oriented mostly on Alzheimer's disease, schizophrenia and depression but very few studies focus on suicidality. We decided, based on the current knowledge, to study suicidal behaviour with the use of proteomics to compare cerebrospinal fluid and platelets. We hypothesized that the same protein group can be detected in pathways that are part of platelet degranulation process in the platelet proteome and cerebrospinal fluid proteome. Based on these findings we suppose, that with use of proteomic analysis a specific protein (group of proteins) can be identified in both, cerebrospinal fluid and platelet proteome in patients with suicidal behavior.Group of proteins identified in our sample in the reactome pathway database (release of platelet secretary granule components and exocytosis of platelet granule contents) supports the idea of link between central nervous system and platelets (“the periphery”). Further research is needed to clarify whether the identified group of proteins taking part in platelet pathways can be used as peripheral biomarkers for suicidal behavior.Disclosure of interestThe authors have not supplied their declaration of competing interest.