Phosphorus (P) is an essential macronutrient for plant growth and is mainly present in agricultural soil in unavailable forms. Phosphate-solubilizing microorganisms (PSMs) increase soil P availability. The objective of the present study was to assess the population and type of PSMs and their relationships with soil characteristics in the agricultural soil of Manokwari. Twenty-one composite soil samples (0–20‍ ‍cm) were collected at the rhizospheres of plants in the Prafi and Masni Districts. A dilution technique and plate count method on Pikovskayas agar medium were used to examine the PSM population, phosphate-solubilizing index (PSI), and various soil properties. The results obtained showed that the total population of phosphate-solubilizing bacteria ranged between 25×10 3 and 550×10 3 CFU g –1 of soil, while that of phosphate-solubilizing fungi was between 2.0×10 3 and 5.0×10 3 CFU g –1 of soil at all locations. The PSI of the isolates ranged between 1.1 to 3.6‍ ‍mm, with the most efficient and highest PSI being obtained for Bacillus sp. (strain 8) and the lowest for Pseudomonas sp. (strain 15). Six isolates found at all locations were identified at the genus level: Chromobacterium sp., Pseudomonas sp., Bacillus sp., Micrococcus sp., Caulobacter sp., and Aspergillus sp. A correlation was observed between the number of PSMs and the level of soil P availability and moisture content, indicating an increase in soil P availability with a greater abundance of PSMs in soil.

Penelitian ini bertujuan untuk menguji aktivitas antibakteri serta mengidentifikasi kandungan metabolit sekunder dari ekstrak metanol buah hitam (H.monticola). Ekstrak buah hitam diperoleh melalui metode ekstraksi maserasi menggunakan pelarut metanol. Hasil skrining fitokimia menunjukkan bahwa ekstrak metanol buah hitam (H.monticola) mengandung tanin dan saponin dengan kadar kategori tinggi. Uji aktivitas antibakteri dilakukan menggunakan metode sumuran dengan kontrol positif kloromfenikol dan kontrol negatif metanol. Hasil uji aktivitas antibakteri terhadap bakteri E.coli menunjukkan aktivitas kuat dengan diameter zona hambat sebesar 11 mm dan bakteri B.subtilis menunjukkan aktivitas sedang dengan zona hambat dengan diameter sebesar 8 mm.

Skin and submental glands of Papuan frogs have antibacterial compounds‘ which were capable to impede growth of tested bacteria based on diffusion method. These frogs were Platymantis papuensis, Litoria infrafrenata, Bufo melanostictus, Rana grisea and Rana sp., and the tested bacterial were the positive Gram bacteria (Staphylococcus aereus, and Bacillus subtilis) and the negative Gram bacteria (Escherichia coli and Pseudomonas aeroginosa). Skin frog which extracted with hot water (100oC) showed antibacterial activity which significantly impeded the growth of tested bacteria using diffusion method.

Copper is an essen al macronutrient for living organisms. Nevertheless, at high concentra ons, it is toxic to most forms of life, including microorganisms. In this research, we examined the biofilm forma on ability and iden fied the molecular characteris cs of copper-resistant bacteria isolated from PT Freeport Indonesia's tailings. Four bacteria isolates from PT Freeport Indonesia's tailings were used in this study. Qualita ve analysis of biofilm forma on by copper-resistant bacteria was performed using the Scanning Electron Microscopy (SEM) method and Micro ter Plate Biofilm Assay. The results showed that the C53 isolate could be categorized as a strong biofilm former, and three other isolates (C38, C40, and C43) as medium biofilm formers. The iden ty of the selected isolates was based on 16S rRNA gene sequence analysis: C38 isolate had a 99% similarity to Bacillus cereus strain HM85, C43 isolate had a 99% similarity to Bacillus sub lis strain EN16, C40 isolate had a 99% similarity to Lycinibacillus fusiformis strain MB52, and C53 isolate had a 98% similarity to Pseudomonas aeuruginosa strain GGRJ21. The capability of the C53 isolate to form strong biofilm can be exploited in bioremedia on processes aiming to remove copper from tailings.

Kolam air panas War Aremi, adalah kolam Air Panas yang terdapat di Kampung Matatun Distrik Kebar, Papua Barat. Bakteri Termofilik tumbuh pada suhu lebih dari 45oC. Bakteri Termofilik menghasilkan enzim yang bermanfaat dalam dunia industri. Penelitian ini merupakan penelitian eksploratif yang bertujuan untuk mengisolasi bakteri termofilik dari Kolam Air Panas War Aremi di Kampung Matatun, Distrik Kebar, Kabupaten Tambrauw, Papua Barat dengan menngunakan Metode Tuang (Pour Plate) Dari hasil penelitian diperoleh tiga isolat bakteri termofilik dari sampel air panas, dan 4 isolat dari sampel sedimen. Berdasarkan hasil pengecatan gram dan pengamatan morfologi sel, ketujuh isolat bakteri termofilik yang  berhasil diisolasi adalah merupakan kelompok bakteri gram positif dengan morfologi sel berbentuk sel batang. Berdasarkan hasil pewarnaan gram, pengamatan morfologi sel dan karakterisasi biokimia, ketujuh isolat bakteri termasuk genus Bacillus.