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OBJETIVO: Este estudo pretendeu monitorar as condições higiênico-sanitárias em cantinas de escolas públicas do estado de Goiás, investigadas na pesquisa Avaliação e Monitoramento da Qualidade dos Alimentos Oferecidos na Merenda Escolar, Goiás, desenvolvida de 2004 a 2007. MÉTODOS: A partir de 2004, foi realizada pesquisa em escolas públicas de Goiás envolvendo três etapas. Na primeira, de 2004 a 2005, foram diagnosticadas as condições higiênico-sanitárias das cantinas dessas escolas, utilizando-se check list. Na segunda etapa, de 2005 a 2007, promoveram-se ações de intervenção nas escolas com capacitação de pessoal técnico. Na terceira etapa, em 2010, foram monitoradas condições higiênico-sanitárias das cantinas em dezoito escolas participantes da primeira etapa, utilizando-se o mesmo check list a fim de comparar a dimensão higiênico-sanitária da alimentação escolar nos dois momentos. O projeto foi aprovado pelo Comitê de Ética em Pesquisa da Universidade Federal de Goiás. RESULTADOS: As escolas estudadas cumpriram parcialmente as exigências legais, visto que as inadequações dos itens avaliados - higiene pessoal, condições da edificação, equipamentos e utensílios, higiene operacional e processamento - estavam presentes em 44,9% das cantinas investigadas no período de 2004 a 2005, e em 37,1% das avaliadas na investigação atual, o que coloca em risco a qualidade higiênico-sanitária da alimentação produzida. CONCLUSÃO: Comparando as etapas de investigação, verificou-se melhora de conformidade apenas nos itens do bloco higiene operacional. Tais resultados reforçam a necessidade de adequação física das cantinas escolares e de capacitação periódica, com vistas à melhoria permanente na produção da alimentação escolar.

ObjectiveThe objectives of the present study were to determine the prevalence of potentially pathogenic microorganisms that indicate the hygienic and sanitary conditions of human milk samples collected at a Human Milk Bank. MethodsThree hundred and thirty eight (338) samples of human milk collected from a milk bank in a maternity in the municipality of Goiânia, in the state of Goias, Brazil were submitted to microbiological analysis. The latter were plated on McConkey agar according to the type of bacteria. Among the total number of samples collected, 194 consisted of raw milk and the remaining 144 were pasteurized milk.

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The objectives of this study were to characterize Staphylococcus aureus isolated from artisanal and industrialized Minas frescal cheeses, to determine their antimicrobial susceptibility profile as well as the genetic similarity among the isolates. The isolates were also tested for staphylococcal enterotoxin genes and other virulence factors. Fifty-six artisanal raw milk cheeses sold at street fairs and 10 industrialized cheeses commercialized in supermarkets of Goiânia, Goiás, were analyzed. Staphylococcus aureus was confirmed in 19 samples (33.9%) of artisanal cheese by detection of femA gene, in which 29 isolates were obtained. These isolates were submitted to the antimicrobial susceptibility test and classified into 9 different profiles (A-I). Thirteen isolates (44.8) were resistant to penicillin and 3 (10.3) to tetracycline, with 2 (7.4) resistant to both. The multiplex PCR technique was performed to detect virulence genes that code for the production of hemolysins (Hla and Hlb), toxic shock syndrome toxin (TSST-1), exfoliative toxins (ETa and ETb), and staphylococcal enterotoxins [SE; SEA-SEE, SEG-SEJ, SEM-SEO]. All the isolates amplified for the hla gene and 14 (48.3%) for the hlb gene. The seh gene was the most frequently detected (n=11, 37.9%), followed by seo gene (n=3; 10.3%). In one isolate (3.4%), 4 enterotoxins genes were detected, and in another, 6 (3.4%) were detected. The comparison performed by pulsed-field gel electrophoresis of the 29 isolates revealed 18 genotypic profiles, which were grouped into 5 clusters. The genotyping found high genetic similarity among the isolates. Identical isolates were obtained from different samples and one sample showed more than one genetically different isolate. The high prevalence of S. aureus in the Minas Frescal cheese samples, as well as the detection of toxin encoding genes identified in this study, warns of the necessity to reduce the contamination levels in this type of cheese through monitoring and controlling the production and trade of the product.