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Crystalline carbonated hydroxyapatite ESD-coated implants positively influenced the biological performance compared with Ti control implants.

This study was designed to examine the influence of integrin subunit-b1 and subunit-b3 on the behavior of primary osteoblast-like cells, cultured on calcium phosphate (CaP)-coated and non coated titanium (Ti). Osteoblast-like cells were incubated with specific monoclonal antibodies against integrin-b1 and integrin-b3 to block the integrin function. Subsequently, cells were seeded on Ti discs, either non coated or provided with a 2 lm carbonated hydroxyapatite coating using Electrostatic Spray Deposition. Results showed that on CaP coatings, cellular attachment was decreased after a pre-treatment with either anti-integrin-b1 or anti-integrin-b3 antibodies. On Ti, cell adhesion was only slightly affected after a pre-treatment with anti-integrin-b3 antibodies. Scanning electron microscopy showed that on both types of substrate, cellular morphology was not changed after a pre-treatment with either antibody. With quantitative PCR, it was shown for both substrates that mRNA expression of integrin-b1 was increased after a pre-treatment with either anti-integrin-b1 or anti-integrin-b3 antibodies. Furthermore, after a pre-treatment with either antibody, mRNA expression of integrin-b3 and ALP was decreased, on both types of substrate. In conclusion, osteoblast-like cells have the ability to compensate to great extent for the blocking strategy as applied here. Still, integrin-b1 and b3 seem to play different roles in attachment, proliferation, and differentiation of osteoblast-like cells, and responses on CaP-coated substrates differ to non coated Ti. Furthermore, the influence on ALP expression suggests involvement of both integrin subunits in signal transduction for cellular differentiation.