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We describe 46 insertions of the Streptococcusfaecalis transposon Tn.917 into the chromosome of Bacillus subtilis. These insertion mutations were mapped genetically. Some caused auxotrophic requirements, and others were cryptic. These insertions were scattered around the B. subtilis chromosome. The We collected a number of such Tn917 insertions and (with the help of the Cornell University Biological Sciences 487 classes of 1982, 1983, 1984, and 1985)

Two conserved histidine residues (His-101 and His-271) appear to be essential components in the active site of the enzyme xylose (glucose) isomerase (EC 5.3

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