The ability to detect early molecular responses to various chemicals is central to the understanding of biological impact of pollutants in a context of varying environmental cues. To monitor stress responses in a model plant, we used transgenic moss Physcomitrella patens expressing the b-glucuronidase reporter (GUS) under the control of the stress-inducible promoter hsp17.3B. Following exposure to pollutants from the dye and paper industry, GUS activity was measured by monitoring a fluorescent product.Chlorophenols, heavy metals and sulphonated anthraquinones were found to specifically activate the hsp17.3B promoter (within hours) in correlation with long-term toxicity effects (within days). At mildly elevated physiological temperatures, the chemical activation of this promoter was strongly amplified, which considerably increased the sensitivity of the bioassay. Together with the activation of hsp17.3B promoter, chlorophenols induced endogenous chaperones that transiently protected a recombinant thermolabile luciferase (LUC) from severe heat denaturation. This sensitive bioassay provides an early warning molecular sensor to industrial pollutants under varying environments, in anticipation to long-term toxic effects in plants.Because of the strong cross-talk between abiotic and chemical stresses that we find, this P. patens line is more likely to serve as a direct toxicity bioassay for pollutants combined with environmental cues, than as an indicator of absolute toxicity thresholds for various pollutants. It is also a powerful tool to study the role of heat shock proteins (HSPs) in plants exposed to combined chemical and environmental stresses.

From the perspective of a circular economy that prioritizes resource reuse and recovery, sewage sludge could be a source of nutrients for agricultural soils or a source of energy, depending on its characteristics. Lombardy is the region with the highest quantity of sludge production and management in Italy. A methodology was developed to: extract and analyze quantitative data on sewage sludge (EWC 190805) production and management (2017–2018); collect and analyze qualitative data from publicly available documents in tender processes for sewage sludge management (2014–2020). Sludge from Lombardy’s wastewater treatment facilities displayed average qualities that were useful for recovery in agriculture after additional stabilization treatments. Sludge showed generally low heating values and elevated water content and should require additional treatments to be used in mono-combustion. The study discovered that there is still work to be done in sludge recovery in agriculture in Lombardy, taking biosolid quality into account. Sludge, on the other hand, can be converted into energy. The methodology for collecting and analyzing site-specific data presented here can be applied to other areas. The findings can assist and guide decision-makers in developing future regional sewage sludge management strategies.

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Jerricans, commonly used to store household drinking water, are challenging to clean and biofilms may develop inside containers storing contaminated water. A large-scale laboratory experiment was conducted to test methods to inhibit biofilm growth.