One of the plants that is well known in the community and is used as a traditional medicine is patchouli (Pogostemon cablin Benth.). It contains essential oils, flavonoids, saponins, tannins, glycosides, terpenoids and steroids. The alcohol content, such as patchouli alcohol and its derivatives, phenol, and a group of terpenoids in patchouli oil has antibacterial activity. The aim at this research to determine the total phenolic condition the ethanol extract from the patchouli leaves. The extract obtained was analyzed ist content using UV-Vis spectrophotometer. The measurements were done at the maximum wavelength of 662,85 nm. The result showed that the total phenolic in patchouli leave is 327,84 mgGAE /gram of extract.
This research aimed to analyze the levels of vitamin C and β-carotene in Moringa leaves extract (Moringa oleifera Lam.) using UV-Vis spectrophotometry. Vitamin C on Moringa leaves are extracted with ethanol 96%. The extracts obtained was qualitatively analyzed of levels of vitamin C using specific reagents an iodine 1% and ammonium molybdate 5%. Than quantitatively using UV-Vis spectrophotometry at wavelength of 570 nm, obtained an verage grade of 7,96 mg/g. For β-carotene, Moringa leaves were extracted with acetone. The extract obtained was extracted again with ether, then saponified with KHO solvent 15% w/v in methanol, allowed to stand overnight. The extract obtained was extracted again with ether, then freed from wet with distilled water. Extracts were analyzed qualitatively using thin layer chromatography and quantitatively using UV-Vis spectrophotometry at wavelength of 450 nm and obtained an average grade of 3,31 mg/g.
The village of Borisallo is a Development Partner Village of UMI in Gowa Regency. One of the educational facilities is SD Inpres Borisallo where a school is a place of learning that can be a threat of disease transmission if it is not managed properly. Problems with children's health behavior are usually related to personal and environmental hygiene. By that, the planting of values of health behavior in elementary schools is an absolute necessity and an effort to realize "Healthy and Independent Community" as contained in the health profile of Gowa Regency. As for students in SD Inpres Borisallo as many as 102 children with 9 teaching staff. Ideally, the health of elementary school children is monitored by UKS, but due to various limitations, the role of the UKS SD Inpres Borisallo has not been implemented optimally and there is no special hand washing place for students. The target to be achieved is to increase students' knowledge, awareness, willingness and ability to live clean and healthy lives, and be expected to play an active role in realizing optimal health degrees, instilling clean and healthy behavioral values (PHBS), improving the practice of handwashing with soap true and became a habit in the daily lives of SD Inpres Borisallo students. The approach taken to achieve the target is to provide clean and healthy life behavior counseling (PHBS) and socialization of how to wash hands with soap properly and correctly, procure posters on how to wash hands and procure water tap pipe installation infrastructure for hand washing needs in SD Inpres Borisallo students.
Chronic exposure to ultraviolet light from the sun will cause changes in skin structure and oxidative stress on the skin. The effects can be in the form of acute changes such as erythema, pigmentation and photosensitivity, as well as long-term effects of premature aging and skin damage. Sun Protection Factor (SPF) is a universal indicator that explains the effectiveness of a product or substance that is UV protection. Natural ingredients that can be used as UV protection are button mushrooms (Agaricus bisporus). This study aims to determine UV light protection activity in the ethanol extract of button mushrooms (Agaricus bisporus) based on the SPF values analyzed by UV-Vis spectrophotometry at wavelengths of 290-320 nm. The results showed that at concentrations of 1% and 2% had a medium SPF category with SPF values of 17,762 and 28,643. For concentrations of 3% and 4% it has a high SPF category with SPF values of 30,592 and 31,325. The higher the concentration used, the better the SPF value.
Pomelo citrus (Citrus maxima (Burm) Merr) is a fruit plant that has the potential to be developed for health. Pomelo fruit contains lycopene, flavonoids and vitamin C which can provide antioxidant activity. The purpose of this study was to determine the potential antioxidant activity of ethanol extract and fraction of n-hexane of pomelo citrus using quercetin as a comparison. Extraction of pomelo citrus fruit using the maceration method with liquid-liquid fractionation using water and n-hexane (1: 3 v / v). Testing of antioxidant activity using the DPPH (1,1-Diphenyl-2-picrylhidrazil) method is based on the ability of the sample to capture DPPH free radicals which are characterized by purple to yellow discoloration and a decrease in the absorbance value of DPPH which has reacted with samples in certain concentrations. Absorbance measurements using a UV-Vis spectrophotometer at a wavelength of 514,942 nm. The results showed that the IC50 value of quercetin was 6.55 µg / mL which was a strong antioxidant, while the IC50 value of the ethanol extract of pomelo citrus fruit was 2882.26 µg / mL and IC50 value of the n-hexane fraction of pomelo orange was 3668,44 µg / mL which is a weak antioxidant category. Kata kunci : Antioxidants, Ethanol Extract, n-Hexane Fraction, Pomelo citrus (Citrus maxima (Burm) Merr
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