Male infertility comprises half of the infertility cases (Miamoto, Minase, Okabe, Ueda & Sengoku, 2015). Various factors including problems in sperm production, maturation and fertilisation ability can affect male reproductive health, leading to male infertility. According to the WHO guidelines (World Health Organization, 2010), semen parameters such as the number, motility and morphology are regarded as reliable predictors of fertility. However, the assessment of these factors may be not sufficient for the diagnosis of infertility, as there are infertile men with a normal spermiogram (Brito, Barth, Bilodeau-Goeseels, Panich, & Kastelic, 2003). Therefore, it seems important to consider other sperm parameters for a clinical diagnosis of infertility. Acrosome reaction plays a critical role in sperm penetration into the oocyte and its fertilisation ability (Jin & Yang, 2017). Since this process

Background: Coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may lead to the infertility of men. Objectives: This study aimed to investigate the impact of COVID-19 infection on sperm parameters and reproductive hormones in fertile men. Methods: A total of 100 males were selected and divided into two groups: (1) patients in convalescence (patients suffering from COVID-19 infection in pharyngeal swab in accordance with reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); (2) negative control group (without antibodies). Semen and blood samples were gathered from all subjects. In the native semen, immunoglobulin (Ig) M and IgG antibodies in the blood were confirmed, and COVID-19 was detected via RT-PCR. To this end, based on the World Health Organization (WHO) guidelines, semen analysis, total antioxidant capacity (TAC), and sperm DNA integrity were assessed. Results: Results demonstrated that sperm concentration, motility, sperm viability, and TAC significantly reduced in fertile males with virus infection. In comparison with the control group, sperm DNA integrity was significantly increased (P < 0.05). Data indicated that the semen volume was not significantly correlated with COVID-19, and there was a significantly negative correlation between sperm concentration, sperm total motility, sperm vitality, sperm normal forms, and TAC with COVID-19. Sperm DNA fragmentation index had a significant and positive correlation with COVID-19 (P < 0.05). In addition, reproductive hormones significantly reduced in fertile males with COVID-19 infection (P < 0.05). Conclusions: COVID-19 infection has a negative influence on sperm parameters and reproductive hormones in fertile males.