Current coronavirus disease-2019 (COVID-19) pandemic has caused massive loss of lives. Clinical trials of vaccines and drugs are currently being conducted around the world; however, till now no effective drug is available for COVID-19. Identification of key genes and perturbed pathways in COVID-19 may uncover potential drug targets and biomarkers. We aimed to identify key gene modules and hub targets involved in COVID-19. We have analyzed SARS-CoV-2 infected peripheral blood mononuclear cell (PBMC) transcriptomic data through gene coexpression analysis. We identified 1520 and 1733 differentially expressed genes (DEGs) from the GSE152418 and CRA002390 PBMC datasets, respectively (FDR < 0.05). We found four key gene modules and hub gene signature based on module membership (MMhub) statistics and protein–protein interaction (PPI) networks (PPIhub). Functional annotation by enrichment analysis of the genes of these modules demonstrated immune and inflammatory response biological processes enriched by the DEGs. The pathway analysis revealed the hub genes were enriched with the IL-17 signaling pathway, cytokine–cytokine receptor interaction pathways. Then, we demonstrated the classification performance of hub genes (PLK1, AURKB, AURKA, CDK1, CDC20, KIF11, CCNB1, KIF2C, DTL and CDC6) with accuracy >0.90 suggesting the biomarker potential of the hub genes. The regulatory network analysis showed transcription factors and microRNAs that target these hub genes. Finally, drug–gene interactions analysis suggests amsacrine, BRD-K68548958, naproxol, palbociclib and teniposide as the top-scored repurposed drugs. The identified biomarkers and pathways might be therapeutic targets to the COVID-19.

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COVID-19 has emerged as global health threats. Chronic kidney disease (CKD) patients are immune-compromised and may have a high risk of infection by the SARS-CoV-2. We aimed to detect common transcriptomic signatures and pathways between COVID-19 and CKD by systems biology analysis. We analyzed transcriptomic data obtained from peripheral blood mononuclear cells (PBMC) infected with SARS-CoV-2 and PBMC of CKD patients. We identified 49 differentially expressed genes (DEGs) which were common between COVID-19 and CKD. The gene ontology and pathways analysis showed the DEGs were associated with “platelet degranulation”, “regulation of wound healing”, “platelet activation”, “focal adhesion”, “regulation of actin cytoskeleton” and “PI3K-Akt signalling pathway”. The protein-protein interaction (PPI) network encoded by the common DEGs showed ten hub proteins (EPHB2, PRKAR2B, CAV1, ARHGEF12, HSP90B1, ITGA2B, BCL2L1, E2F1, TUBB1, and C3). Besides, we identified significant transcription factors and microRNAs that may regulate the common DEGs. We investigated protein-drug interaction analysis and identified potential drugs namely, aspirin, estradiol, rapamycin, and nebivolol. The identified common gene signature and pathways between COVID-19 and CKD may be therapeutic targets in COVID-19 patients with CKD comorbidity.

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The recent two decades have witnessed tremendous growth in Internet of things (IoT) applications. There are more than 50 billion devices connected globally. IoT applications’ connectivity with the Internet persistently victimized them with a divergent range of traditional threats, including viruses, worms, malware, spyware, Trojans, malicious code injections, and backdoor attacks. Traditional threats provide essential services such as authentication, authorization, and accountability. Authentication and authorization are the process of verifying that a subject is bound to an object. Traditional authentication and authorization mechanisms use three different factors to identity a subject to verify if the subject has the right capability to access the object. Further, it is defined that a computer virus is a type of malware. Malware includes computer viruses, worms, Trojan horses, spyware, and ransomware. There is a high probability that IoT systems can get infected with a more sophisticated form of malware and high-frequency electromagnetic waves. Purpose oriented with distinct nature IoT devices is developed to work in a constrained environment. So there is a dire need to address these security issues because relying on existing traditional techniques is not good. Manufacturers and researchers must think about resolving these security and privacy issues. Most importantly, this study identifies the knowledge and research gap in this area. The primary objective of this systematic literature review is to discuss the divergent types of threats that target IoT systems. Most importantly, the goal is to understand the mode of action of these threats and develop the recovery mechanism to cover the damage. In this study, more than 170 research articles are systematically studied to understand security and privacy issues. Further, security threats and attacks are categorized on a single platform and provide an analysis to explain how and to what extent they damage the targeted IoT systems. This review paper encapsulates IoT security threats and categorizes and analyses them by implementing a comparative study. Moreover, the research work concludes to expand advanced technologies, e.g., blockchain, machine learning, and artificial intelligence, to guarantee security, privacy, and IoT systems.