Facial cream preparation contains active fotoprotector, antioxidant, and moisturizer derived fromnatural sources. Cocoa beans and honey bee are ones of anti-oxidant and moisturizer natural sourcescontaining polyphenol/phenolic and other flavonoid compounds. Flavonoid contains anti-oxidant agent asfree radical scavenger. The aim of this study was to obtain formula combination of non-fermented cocoabeans methanol extract and honey bee as active agents in effecting face skin moisturizing. Phases of theresearch consists of process of producing non-fermented cocoa beans methanol extract, producing facecream from non-fermented cocoa beans methanol extract and honey bee, and analysis on the characteristicsof the products. The result showed that face cream formulated from 1.56% of non-fermented cocoa beansmethanol extract and 2.34% of honey bee having 73.8% moisturizing effect in 60 minutes exposure doesnot irritate face skin and eyes. It has viscosity value 2.72 x 105 cps, has pH value 5.26 which is suitable withthe range of normal pH for skin (4.5-7.5), does not contain residues of nipasol and nipagin, has scoringaverage 1 – 2.9 by panelists on the attributes (aroma, consistency, texture, stickiness, homogeneoussensation, color, appearance, itchiness, erythema, easiness on cleaning, response on washing), and hastotal bacteria, khamir, and molod 5.0 x 102 which does not meet the requirements for skin moisturizer (SNI16-4339-1996) i.e. 102 colonies/gram.Keywords: non-fermented cocoa beans, honey bees, the active component, facial cream

Research on the effect of formulation and fermented cocoa beans origin to dark chocolate’s quality and flavour has been done. This research aims to know how is the effect of formulation and fermented coco beans origin to the quality and flavours of resulted dark chocolate. This research used 2 (two) treatment . First treatment was origin of coco beans used on the research (A), in which : South Sulawesi (A1), West Sulawesi (A2), Central Sulawesi (A3), second treatment was formula quality of dark chocolate to be produced (B), in which medium quality (B1) and high quality  formula (B2). Testing parameter used were water moisture, fat content, sucrose content, FFA, fatty acid, amino acid, polyphenol, flavour, TPC and melting point. Research  result  can  be  concluded  that formulation  and  origin  of  fermented  cocoa  beans affecting quality and flavourof resulted dark chocolate in tenns of melting point, amino acids content, fatty acids, polyphenol content, and flavour score. Dark chocolate produced Central Sulawesi both for medium quality and high quality had higher polyphenol content compare to those from both South and West Sulawesi.Keywords : Cocoa beans, fermentation, dark chocolate, medium quality, high quality, flavour.ABSTRAK Penelitian Pengaruh Formulasi Dan Asal Biji Kakao Fermentasi Terhadap Mutu Dan Citarasa Dark Chocolate telah dilakukan. Penelitian ini bertujuan untuk mengetahui sejauh mana pengaruh formulasi da asal biji kakao fermentasi  terhadap mutu dan citarasa dark chocolate yang dihasilkan. Pada penelitian pembuatan Dark Chocolate menggunakan 2 (dua) perlakuan . Perlakuan pertama yaitu Asal Daerah (provinsi) biji kakao yang digunakan (A) yaitu ; Sulawesi Selatan (A1), Sulawesi Barat (A2), Sulawesi Tengah (A3), dan perlakuan ke dua adalah formula/kualitas Dark Chocolate yang akan di buat (B), yaitu Formula Kualitas sedang (B1), dan Formula Kualitas Tinggi (B2). Parameter uji yang digunakan adalah kadar air, kadar lemak, kadar gula, FFA, asam lemak, asam amino, polifenol, citarasa, ALT dan titik leleh. Dari hasil penelitian dapat disimpulkan bahwa formulasi dan asal biji kakao fermentasi mempengaruhi mutu dan citarasa dark chocolate yang dihasilkan terutama dari segi  titik leleh, kadar asam amino, asam lemak, kadar polifenol dan nilai citarasa. Dark chocolate yang dihasilkan  dari kakao  asal  Sulawesi  tengah  baik    kualitas  sedang  maupun  kualitas  tinggi memiliki kandungan polifenol tertinggi dibanding dark chocolate dari Sulawesi Selatan dan Sulawesi Barat.Kata Kunci : Biji kakao, fermentasi, dark chocolate, kualitas sedang, kualitas tinggi, mutu, citarasa.

Cocoa liquor and powder are food ingredients that contain many functional compounds such as polyphenols which are very useful for health. This research aims to determine the characteristics and effects of functional foods from cocoa liquor and powder on blood cholesterol levels. The research methodology used experimental and analytical methods by making cocoa paste and cocoa powder from unfermented cocoa beans with the addition of 5% sucrose. Two treatments were performed on functional food samples: functional food in the form of cocoa liquor (A3) and powder (B3). Results show that functional food of cocoa liquor (A3) has characteristics as followed: 16.

Epidermis of cocoa beans contain active compounds that are similar to the active compound in the cocoa pod husk. Epidermis of cocoa beans are suspected to contain active compounds such as  polyphenols, flavonoids, terpenoids/steroids, condensed or polymerized tannins such as catechins and anthocyanins. The bioactive compounds were known to have antibacterial properties on Streptococcus mutans types. The aim of this research were to produce natural active compounds from cocoa bean husk, which have antibacterial activity against Streptococcus mutans. Stages of the study consisted of powdering process of epidermis cocoa beans, extracting fat from epidermis cocoa beans powder, extracting of the antibacterial active compound, solvent evaporation of active compounds extracts from powdered cocoa beans husk, and testing of active compounds produced by Streptococcus mutans. Results showed that the minimum inhibitory concentration (MIC) of the cocoa bean husk extract againsts Streptococcus mutans on a half-day incubation period was 0.25% with an inhibitory diameter of 7.550 mm. While, a two- days incubation period was 0.25% with an inhibitory diameter of 9,900 mm and was bacteriostatic.Keywords: cocoa beans husk, maceration extraction, methanol, antibacterial, active components, Streptococcus mutans ABSTRAK. Kulit ari biji kakao mengandung senyawa aktif yang tidak berbeda jauh dengan kandungan senyawa aktif yang terdapat pada kulit buah kakao dan biji kakao itu sendiri. Kulit ari biji kakao diduga mengandung senyawa aktif polifenol, flavonoid, terpenoid/steroid, tanin terkondensasi atau terpolimerisasi seperti katekin dan antosianin. Senyawa-senyawa bioaktif tersebut diketahui memiliki sifat antibakteri jenis Streptococcus mutans.Tujuan penelitian ini adalah pemanfaatan kulit ari biji kakao sebagai sumber senyawa aktif yang memiliki aktivitas antibakteri terhadap bakteri Streptococcus mutans. Tahapan penelitian terdiri dari proses pembuatan bubuk kulit ari biji kakao, proses ekstraksi lemak dari bubuk kulit ari biji kakao, proses ekstraksi senyawa aktif antibakteri dari bubuk kulit ari biji kakao, proses penguapan pelarut ekstrak senyawa aktif dari bubuk kulit ari biji kakao, dan proses pengujian senyawa aktif yang dihasilkan terhadap Streptococcus mutans. Hasil penelitian menunjukkan bahwa ekstrak limbah kulit ari biji kakao berpotensi sebagai senyawa aktif antibakteri terhadap Streptococcus mutans. Konsentrasi hambat minimum (KHM) ekstrak kulit ari biji kakao terhadap bakteri Streptococcus mutans pada masa inkubasi satu setengah hari adalah 0,25% dengan diameter hambatan sebesar 7,550 mm dan masa inkubasi dua hari adalah 0,25% dengan diameter hambatan sebesar  9,900 mm dan bersifat bakteriostatik.  Kata kunci : kulit ari biji kakao, ekstraksi maserasi, metanol, antibakteri, komponen aktif, Streptococcus mutans

This study aims to determine the content of amino acids and fatty acids in unfermented cocoa powder with low temperature steam roasting treatment. The experimental research method was the processing technology of flavonoid-rich cocoa powder with the blanching method with steaming (hot steam), drying cocoa beans, and roasting using the Low Temperature Short Time method. The raw materials used are cocoa pods from the district Bantaeng, South Sulawesi. The parameters analyzed were the amino acid and fatty acid content of roasted and non-roasted cocoa powder, compared to dry cocoa beans using gas chromatography and LCMS methods. The results showed that the highest fatty acid in dry cocoa beans was Methyl Octadecanoate (32.16%), in unroasted and roasted cocoa powder, namely . While the highest amino acid in dry cocoa beans is L-Arginine (1.13%). L-Lysine as the highest amino acid in unroasted and roasted cocoa powder was 6.76% and 4.8%, respectively. For the lowest types of amino acids in cocoa beans and powder, namely L-cysteine in dry cocoa beans (0.001%), unroasted cocoa powder (0.07%) and roasted cocoa powder (0.06%).