Abstrak: Masalah  resistensi bakteri terhadap antibiotik  tidak  hanya terjadi  di  Indonesia tetapi  juga  secara global  dan  menjadi  satu  persoalan yang  cukup  rumit  dan  harus  segera diatasi   bersama-sama. Di masyarakat, kerap kali antibiotik dibeli tanpa penjelasan dan resep dokter. Masyarakat juga kerap membeli antibiotik dengan resep yang pernah diperoleh sebelumnya, bahkan langsung mengonsumsi antibiotik untuk penyakit ringan yang penanganannya tidak selalu membutuhkan antibiotik seperti demam, batuk dan pilek. Tujuan dari kegiatan pengabdian masyarakat ini ialah memberikan edukasi mengenai bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional kepada masyarakat desa Air salobar kecamatan Nusaniwe Ambon. Metode yang digunakan pada kegiatan ini adalah metode ceramah dengan memberikan materi mengenai bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional secara langsung. Hasil dari kegiatan pengabdian yang telah dilakukan menunjukkan bahwa masyarakat desa Air Salobar sangat antusias terhadap kegiatan yang dilaksanakan. Hal tersebut ditandai dengan antusiasnya, warga dalam menerima materi edukasi. Selain itu, warga yang hadir dalam kegiatan ini aktif memberikan respon timbal balik terhadap pemateri dengan cara memberikan pertanyaan-pertanyaan mengenai penyebab dan efek dari resistensi bakteri terhadap antibiotik. Abstract: Bacterial resistance issue does not only occur in Indonesia but also globally and becomes a quite complex problem and must be addressed together. Commonly, antibiotics are often purchased without a doctor’s explanation and prescription. People tend to buy antibiotics with prescriptions that have been obtained before, even directly consuming antibiotics for minor ailments whose treatment does not always require antibiotics such as fever, cough and cold. The purpose of this activity was to educate the community on the dangers of bacterial resistance due to irrational use of antibiotics to the community of Air Salobar Village, Nusaniwe district, Ambon. The method used was a lecture method by providing material on the dangers of bacterial resistance due to irrational use of antibiotics directly. The results showed that the residents of Air Salobar village are very enthusiastic about the community service activity carried out. This was indicated by the enthusiasm of the residents in receiving educational materials. Apart from that, the residents who attended this activity actively gave a reciprocal response to the presenters by asking questions about the causes and effects of bacterial resistance to antibiotics.

Penyakit infeksi pada masyarakat Indonesia khususnya di Provinsi Maluku, Ambon masih menjadi masalah kesehatan masyarakat yang penting untuk diperhatikan. Penyakit infeksi ini bisa diobati dengan menggunakan antibiotik. Namun penggunaan antibiotik yang tidak rasional dapat menimbulkan penyakit baru pada penderitanya. Tujuan dari penelitian ini adalah untuk menganalisis tingkat pengetahuan masyarakat Desa Air Salobar terhadap bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional. Metode yang digunakan adalah metode deskriptif yaitu dengan memberikan pretest dan posttest kepada masyarakat Desa Air Salobar RT / RW 003/005 Kecamatan Nusaniwe Ambon. Hasil pengabdian masyarakat bahwa baik responden perempuan maupun laki-laki menunjukkan adanya peningkatan pengetahuan tentang bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional. Responden perempuan mengalami peningkatan nilai sebesar 20,55% dari 18 responden, sedangkan untuk responden laki-laki terjadi peningkatan nilai sebesar 32,85% dari 7 responden. Total rata-rata tingkat peningkatan pengetahuan tentang bahaya resistensi bakteri akibat penggunaan antibiotik yang tidak rasional adalah 24% dari total jumlah responden.

Aims: Cricula trifenestrata is one of natural insects which has not been domesticated yet, thus called as the wild silkworm. C. trifenestrata is known as a silk producer which has high economic and market value. However, the fungi attack on C. trifenestrata cocoon decreased quality and quantity of silk yarn. Chitinolytic bacteria have a high potential as biological control against pathogenic fungi. This research aimed to isolate, select, characterize, and identify chitinolytic bacteria as pathogenic fungi growth inhibitors on C. trifenestrata cocoon. Methodology and results: Chitinolytic bacteria was isolated from the uninfected and infected cocoon while fungi was isolated from the uninfected cocoon. Inhibition test was conducted by Fokkema method and chitinase activity was measured by Spindler method. A total of 36 chitinolytic bacteria and 10 suspected pathogenic fungi isolates have been isolated. Fungal pathogenicity test showed that isolate CSAJ.2 was suspected as fungal pathogen. In vitro inhibition test indicated that chitinolytic bacteria isolate BSEP.3 could inhibit the growth of pathogenic fungi CSAJ.2 with percentage of inhibition 50%. Isolate BSEP.3 showed highest chitinase activity (5.11 U/mL) at the 15 th h. It able to inhibit the growth of pathogenic fungi with percentage of inhibition of 47.5% and 46.25%, respectively. Conclusion, significance and impact of study: Identification of bacteria targeted on 16S rRNA gene showed that isolate BSEP.3 had 98% identity with Bacillus amyloliquefaciens B5 while identification of fungi using ITS region of the rDNA showed that isolate CSAJ.2 had 100% identity with Trichoderma virens TV242. Chitinase crude extract was effective to be used as a biological control agent of T. virens CSAJ.2.

Abstrak: Serangan dari wabah novel coronavirus (Covid-19) telah menyebar secara masiv di seluruh wilayah Indonesia dan pernah menjadi kasus dengan angka kematian tertinggi di Asia tenggara. Berbagai upaya telah dilakukan oleh pemerintah bersama dengan pihak terkait untuk mencegah transmisi Covid-19 yang sangat cepat, termasuk dengan mempromosikan perilaku hidup bersih dan sehat (PHBS) pada seluruh lapisan masyarakat, khususnya di Desa Air Salobar Ambon. Tujuan dari kegiatan ini adalah untuk menganalisis level pengetahuan masyarakat mengenai PHBS untuk mencegah transmisi Covid-19. Kegiatan ini dilaksanakan pada 20 Maret 2021 di Desa Air Salobar dengan melibatkan 26 responden. Metode yang digunakan pada kegiatan ini ialah metode deskriptif yaitu dengan memberikan pretest, edukasi berupa penyuluhan PHBS, dan posttest kepada masyarakat. Hasil analisis menunjukkan bahwa nilai posttest meningkat dari 40,8% menjadi 95% setelah diberikan edukasi dengan rata-rata peningkatan nilai posttest dibandingkan pretest ialah sebesar 56.2%. Dapat disimpulkan bahwa edukasi membrikan dampak yang signifikan terhadap peningkatan level pengetahuan masyarakat mengenai PHBS setelah mendapat edukasi dari fasilitator.Abstract: The attack from novel coronavirus (Covid-19) outbreak has spread massively throughout Indonesia and has ever become the case with the highest death rate in Southeast Asia. Various efforts have been conducted by the government together with related parties to prevent the very rapid transmission of Covid-19, including by promoting clean and healthy living behavior (PHBS) at all levels of society, particularly in Air Salobar Village, Ambon. The objective of the community service activity was to analyze the level of knowledge of the community on PHBS to prevent the transmission of Covid-19. This activity was carried out on March 20, 2021 in Air Salobar Village by involving 26 respondents. The method used was a descriptive method, by providing pretest, education in the form of PHBS counseling, and posttest to the respondents. The results of the analysis showed that the posttest score increased from 40.8% to 95% after being given education with an increase in knowledge level of 56.2%.

Abstract. Sukmawati S, Rosalina F, Sipriyadi, Dewi NK, Yunita M, Sarhan ART, Rahayu Y, Kusumawati E. 2022. Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia. Biodiversitas 23: 1427-1432. The mangrove ecosystem is a producer of detritus and a source of nutrients and organic matter. Some of the ecological functions of mangrove forests are coastline protector, preventing seawater intrusion, as a habitat for various living creatures, a microclimate regulator, a nursery ground, spawning ground, as well as a feeding ground for various aquatic biota. The mangrove forest ecosystem cannot be separated from the role of microbes in helping the process of soil biochemical cycles. In the biochemical cycle, microbes are able to maintain the availability of macronutrients in the soil. The objective of this study was to identify the diversity of bacteria in the mangrove ecosystem in Klawalu, Sorong City. The research method descriptively described the diversity of bacterial species found in the mangrove ecosystem in Klawalu, Sorong City, West Papua Province. The results indicated that the DNA fragments of the four isolates obtained from this study were around 1300 bp. Meanwhile, the bacterial species obtained were isolated SA3, identified as Bacillus safensis strain C251, isolate SA8 identified as Bacillus amyloliquefaciens strain NO10, isolate SL8 was identified as Clostridium sp. JC336, and isolate SL1 was identified as Bacillus australimaris strain IIHR GAPB01.