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IntroductionHigher expression levels of serine/threonine-protein kinase 1 (PLK1) are significantly associated with tumorigenesis and poor clinical prognoses. Consequently, PLK1 is considered a latent target in cancer treatment. We aimed to determine the cytotoxic effects of RO3280 on prostate cancer cells.Material and methodsPLK1 expression was investigated using real-time PCR and western blotting in prostate cancer tissues and paired normal tissues. Real-time cell analysis, cell counting kit-8 assays, and 5-ethynyl-2¢-deoxyuridine cell proliferation assays were applied for the examination of cell proliferation ability. Wound healing assays and transwell assays were used to assess the migratory and invasive abilities of the prostate cancer cell lines with or without RO3280 treatment. Moreover, the target genes and pathways were detected by transcriptomics RNA sequencing in the cells cultured in RO3280 and through a series of bioinformatics analyses. Finally, the Wnt/β-catenin pathway was screened out and verified by western blotting.ResultsWe observed the mRNA and protein overexpression of PLK1 in the prostate cancer cells and tissues. The inhibition of PLK1 by RO3280 significantly reduced the migratory, invasive, and proliferative properties of the RO3280-treated cancer cell lines compared with their controls. RO3280 mediated the inactivation of the Wnt/β-catenin pathway, and reduced the rates of cell proliferation, migration, and invasion in prostate cancer cells.ConclusionsThis study’s findings are significant owing to the identification of the specific anticancer mechanism of RO3280, which may have therapeutic effects. This trial provides clarity on the feasibility of the use of RO3280 as a cancer therapeutic agent for prostate cancer.

Objective Based on the theory of planned behavior, this qualitative study attempted to explore the influence of personal and social levels on prostate cancer screening behavior towards men over 40 years old. Methods Semi-structured interviews were conducted with 21 men over 40 (n = 21) in a health check-up center. The interviews were analyzed using the Collazzi's phenomenological method. Results 57.14% respondents performed PSA test,but almost of them all belong to involuntary and non-autonomous screening. 70% respondents indicated they would screen prostate cancer according to doctors' recommendations. 50% respondents believed that screening should be performed after symptoms appear and emphasized the convenience of including items in the health check-up package. 10% respondents considered screening as an opportunity to obtain psycho-social support. In addition, cognitive impairment and cancer fatalism are obstacles. Of course, external economic conditions and access also have a definite impact. Conclusion This study provided a reference for understanding the complexity and uniqueness of influencing factors of prostate cancer screening willingness in China. As for potential challenges of insufficient prostate cancer screening in China, these findings also provide interventions that can be used to guide prostate cancer screening projects.

Objective To explore the factors influencing the willingness to screen for prostate cancer in middle-aged and elderly men, and to provide a basis for improving the status of insufficient prostate cancer screening in my country, and to improve the overall prognosis and quality of life of prostate cancer patients in my country. Methods Using phenomenological research methods to conduct semi-structured in-depth interviews with 21 middle-aged and elderly men, using MAXQDA software and Colaizzi's seven-step analysis method for coding, analysis and subject extraction. Results Based on the theoretical framework of planned behavior, the influencing factors of the willingness to screen for prostate cancer in middle-aged and elderly men can be refined into three themes of behavior attitude, subjective norms, and perceived behavior control, and 12 sub-themes of its sub-themes. Conclusion The willingness of middle-aged and elderly men to screen for prostate cancer is affected by many factors. Medical and health workers should adopt a variety of comprehensive interventions to guide the reasonable screening of middle-aged and old men to promote their prostate cancer screening behaviors.