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We present a new paradigm for fine-tuning large-scale visionlanguage pre-trained models on downstream task, dubbed Prompt Regularization (ProReg). Different from traditional fine-tuning which easily overfits to the downstream task data, ProReg uses the prediction by prompting the pretrained model to regularize the fine-tuning. The motivation is: by prompting the large model "a photo of a [CLASS]", the fillin answer is only dependent on the pretraining encyclopedic knowledge while independent of the task data distribution, which is usually biased. Specifically, given a training sample prediction during fine-tuning, we first calculate its Kullback-Leibler loss of the prompt prediction and Cross-Entropy loss of the ground-truth label, and then combine them with a proposed sample-wise adaptive trade-off weight, which automatically adjusts the transfer between the pretrained and downstream domains. On various out-of-distribution benchmarks, we show the consistently strong performance of ProReg compared with conventional fine-tuning, zero-shot prompt, prompt tuning, and other state-of-the-art methods.