A new series of transition metal complexes of Cu(II), Ni(II), Zn(II) and VO(IV), were synthesized from the Schiff base (L) derived from 4-aminoantipyrine, 3-hydroxy-4-nitrobenzaldehyde and acetylacetone. The structural features were arrived from their elemental analyses, magnetic susceptibility, molar conductance, Mass, IR, UV-Vis., 1 H NMR and ESR spectral studies. The data show that the complexes have composition of [ML]X type. The UV-Vis., magnetic susceptibility and ESR spectral data of the complexes suggest a square-planar geometry around the central metal ion except for VO(IV) complex which has squarepyramidal geometry. The redox behavior of copper and vanadyl complexes were studied by cyclic voltammetry. The antimicrobial screening tests were also recorded and gave good results in the presence of metal ion in the ligand system. The nuclease activity of the above metal complexes shows that the copper and nickel complexes cleave DNA through redox chemistry, whereas other complexes are not effective.

No abstract

No abstract

The pathogenesis of glycerol-induced myoglobinuric acute renal failure involves ischemia, vascular congestion and reactive oxygen metabolites. In this study, we have investigated for the first time, the role of ferulic acid in attenuating glycerol-induced nephrotoxicity. Male Wistar rats were injected intramuscularly with 8 mL/kg body weight of 50% glycerol, glycerol þ ferulic acid at the dose of 5, 10, 15, 20 and 25 mg/kg body weight. After 24 h, the rats were sacrificed and the kidneys were removed for histological and immunohistochemical studies. Furthermore, determinations of lipid peroxidation (LPO) as well as antioxidant enzymes were also analyzed; blood, urine samples were collected in order to quantify renal function and nitric oxide generation, respectively. Glycerol-induced rats showed a significant increase in the level of urinary markers assessed in serum as well as kidney and these were reversed upon ferulic acid treatment. A significant increase in urine nitric oxide, serum as well as kidney LPO, decrease in activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and reduced glutathione were observed in glycerol-induced rats. Immunohistochemical study in glycerol-induced rats demonstrated an increase in the level of nuclear factor-kappaB (NF-kB). All these effects induced by glycerol were reduced upon treatment with ferulic acid in a dose-dependent manner. To conclude, ferulic acid enhances antioxidants and decreases NF-kB, thereby protecting the cells against stress induced by glycerol.

The bioaccumulation of chromium from retan chrome liquor by Spirulina fusiformis was investigated under laboratory as well as field conditions. At the optimal conditions, metal ion uptake increased with initial metal ion concentration up to 300mg/l. The effect on various physico-chemical parameters like total solids (TS), total dissolved solids (TDS), total suspended solids (TSS), chlorides, sulphates, phenols, biochemical oxygen demand (BOD) and chemical oxygen demand (COD) and biochemical studies related to biomass, chlorophyll-a and protein were also carried out. The present study indicates that S. fusiformis is very effective in removal of chromium (93-99%) besides removing other toxicants from retan chrome liquor. The sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and FTIR studies indicate the interaction/complexation between Cr and alga. The mechanism involved in bioaccumulation of chromium is also discussed. The process when upgraded can be applied for detoxification of tannery effluents.