The purpose of this study was to evaluate various types of fermentor for dry matter digestibility (DMD), organic matter digestibility (OMD), and the chemical composition of fermented seaweed. Five types of seaweed were used as substrates included green strain of Kappaphycus alvarezii, brown strain of K. alvarezii, Gracilaria gigas, Sargassum sp., and Caulerpa sp. The treatments were four fermentors, namely Bacillus sp. 2 mL/100 g of seaweed flour; 1.5% of tape yeast as a source of Rhizopus sp.; 1.5% of baker's yeast as a source of Saccharomyces sp.; a mix of Bacillus sp., tape yeast of Rhizopus sp. and baker's yeast of Saccharomyces sp. with compositions of 1 mL+1 g+1 g/100 g of seaweed flour; and control treatment. The results showed an increase in the percentage of DMD (21.94-27.76%) and OMD (8.35-11.66%) of all seaweed fermented using fermentor compared to control (DMD of 17.65-20.36% and OMD of 4.36-5.98%). Moreover, the highest result was obtained by the fermentor mix (DMD of 24.86-27.76% and OMD of 10.02-11.66%). Similar result was also found in the chemical composition of fermented seaweed, there was increase in protein content of 9.23-15.93% and nitrogen free extract (NFE) of 56.05-70.26% in each seaweed treated with fermentation using fermentors, compared to controls (protein of 8.82-11.54% and NFE of 52.26-65.72%). Furthermore, the highest value was shown by seaweed fermented with mixed fermentors (protein of 9.92-15.93% and NFE of 58.47-70.26%). Yet, the opposite result was present in the ash, crude fiber, and fat content of seaweed fermented using fermentors of which the lowest value was found in treatment of mixed fermentor.Keywords: fermentation, fermentor, seaweed, quality, feed ingredients ABSTRAK Tujuan penelitian ini adalah mengevaluasi berbagai jenis fermentor terhadap kecernaan bahan kering (KBK), kecernaan bahan organik (KBO), dan komposisi kimia rumput laut terfermentasi. Lima jenis rumput laut digunakan sebagai substrat, yaitu Kappaphycus alvarezii strain hijau, K. alvarezii strain coklat, Gracillaria gigas, Sargasum sp., dan Caulerva sp. Perlakuan yang diuji empat fermentor, yaitu Bacillus sp. 2 mL/100 g tepung rumput laut; 1,5% ragi tape sebagai sumber Rhizopus sp.; 1,5% ragi roti sebagai sumber Saccharomyces sp.; campuran Bacillus sp., ragi tape Rhizopus sp., dan ragi roti Saccharomyces sp. dengan komposisi 1 mL+1 g+1 g/100 g tepung rumput laut; serta kontrol. Hasil penelitian menunjukkan terjadi peningkatan persentase KBK (21,94-27,76%) dan KBO (8,35-11,66%) semua jenis rumput laut difermentasi dengan fermentor dibandingkan kontrol (KBK 17,36, dan KBO 4,98%) dan yang tertinggi dengan fermentor campuran (KBK 24,76 dan KBO 10,(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)66%). Begitu juga yang terjadi pada komposisi kimia rumput laut difermentasi, terjadi peningkatan kadar protein (9,23-15,93%) dan bahan ekstrak tanpa nitrogen/BETN (56,05-70,26%) pada setiap rumput laut yang diberi perlakuan fermentasi menggunakan fermentor, dibandingkan kontrol (protein 8,82-11,54% dan BETN 52,26-65,72%), y...

Earthworms meal (Lumbricus sp.) is very prospective as milkfish feed raw materials to substitute fish meal. Type of raw material and the exact composition will generate artificial feed quality with high levels of water stability, desirable, and safe for the fish. The purpose of this study to evaluate the quality of milkfish feed at different levels of fish meal substitution with earthworms (Lumbricus sp.) based on organoleptic, physical, and chemical tests. The treatments tested levels of substitution of fish meal with earthworms meal in artificial feed milkfish, namely: feed A (0%); feed B (34,62%); feed C (65,38%) and feed D (100%). The organoleptic and physical test showed that all the feed has a smooth texture, pungent aroma, and brown in color, with good water stability (rupture velocity ranged from 91,25±1,47 up to 92,87±1,67 minutes and dispersion of solids 11,14±1,55 up to 11,87±1,3%), hardness 84±0,18 up to 84,71±1,24%, sinking velocity 5,07±0,68 up to 5,64±0,17 cm/sec, the level of homogeneity of 81,34±0,17 up to 85,68±1,85%, the allure of 0,62±0,58 up to 0,65±0,12 cm/sec and delicious power of 0,059±0,024 up to 0,067±0,032 g/fish weight/day. The quality of feed is chemically with moisture content ranging from 8,4-9,1%, 16,7-19,46% ash, 31,07-32,37%, protein, 6,67-7,58% fat, crude fiber 7,45-7,87%, NFE (nitrogen free extracts) 35,35-35,48%. Results show that different levels of substitution of fish meal with earthworms meal (Lumbricus sp.) produces the same feed quality and contains nutrients in a range requirement milkfish. Accordingly, earthworms meal (Lumbricus sp.) can be substituted for fish meal in fish milk feed artificial up to 100%.Keywords: substitution, fish meal, earthworms meal (Lumbricus sp.), artificial feed, milkfish ABSTRAKTepung cacing tanah (Lumbricus sp.) sangat menjanjikan sebagai bahan baku pakan ikan bandeng untuk menyubstitusi tepung ikan. Jenis bahan baku dan komposisi yang tepat akan menghasilkan pakan buatan yang berkualitas dengan tingkat water stability yang tinggi, disukai, dan aman bagi ikan. Tujuan penelitian ini mengevaluasi kualitas pakan ikan bandeng pada berbagai tingkat substitusi tepung ikan dengan cacing tanah (Lumbricus sp.) berdasarkan uji organoleptik, fisik, dan kimiawi. Perlakuan yang diuji adalah tingkat substitusi tepung ikan dengan tepung cacing tanah dalam pakan buatan ikan bandeng, yaitu: pakan A 0%; pakan B 34,62%; pakan C 65,38%; dan pakan D 100%. Pengujian organoleptik dan fisik memperlihatkan bahwa semua pakan mempunyai tekstur yang halus, aroma yang menyengat, dan berwarna cokelat, dengan water stability yang baik (kecepatan pecah berkisar antara 91,25±1,47 hingga 92,87±1,67 menit dan dispersi padatan 11,14±1,55 hingga 11,87±1,3%), tingkat kekerasan 84±0,18 hingga 84,71±1,24%, kecepatan tenggelam 5,07±0,68 hingga 5,64±0,17 cm/detik, tingkat homogenitas 81,34±0,17 hingga 85,68±1,85%, daya pikat 0,62± 0,58 hingga 0,65±0,12 cm/detik dan daya lezat 0,059±0,024 hingga 0,067±0,032 g/bobot ikan/hari. Kualitas pakan secara kimiawi dengan kadar air b...

Barramundi (L. calcarifer) is a valuable fish with aquaculture potential. One of the factors determining the success of barramundi aquaculture is the availability of seeds, however larviculture success is often low. This research examined the effect of supplementation with various combinations of vitamin B complex and phosphorus on the RNA/DNA ratio and growth of captive-bred barramundi larvae. Barramundi larvae aged 2 days were stocked at a density of 25 fish/L and reared for 30 days with natural feed (Chlorella and rotifers) in 40 L containers filled with 30 L water. Commercial vitamin B complex and mineral phosphorus were dissolved in water and added to the larval rearing media. The research used a completely randomized factorial design with 2 factors, 4 levels and 3 replicates, giving 16 treatments and 48 experimental units. The factors were vitamin B complex (0, 75, 150, and 225 mg/L) and phosphorus (0, 0.5, 1.0, and 1.5 mg/L). Analysis of variance showed that the combination of vitamin B complex and phosphorus had a highly significant effect (p<0.01) on the RNA/DNA ratio. Barramundi larval growth was lowest with 0 mg/L of both supplements (control) and highest with 150mg/L vitamin B complex and 1.0 mg/L phosphorus, decreasing with higher levels of either additive.