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The inhibition of dipeptidyl peptidase-IV (DPPIV) is a popular route for the treatment of type-2 diabetes. Commercially available gliptin-based drugs such as sitagliptin, anagliptin, linagliptin, saxagliptin, and alogliptin were specifically developed as DPPIV inhibitors for diabetic patients. The use of Gynura bicolor in treating diabetes had been reported in various in vitro experiments. However, an understanding of the inhibitory actions of G. bicolor bioactive compounds on DPPIV is still lacking and this may provide crucial information for the development of more potent and natural sources of DPPIV inhibitors. Evaluation of G. bicolor bioactive compounds for potent DPPIV inhibitors was computationally conducted using Lead IT and iGEMDOCK software, and the best free-binding energy scores for G. bicolor bioactive compounds were evaluated in comparison with the commercial DPPIV inhibitors, sitagliptin, anagliptin, linagliptin, saxagliptin, and alogliptin. Drug-likeness and absorption, distribution, metabolism, and excretion (ADME) analysis were also performed. Based on molecular docking analysis, four of the identified bioactive compounds in G. bicolor, 3-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3,4-dicaffeoylquinic acid, and trans-5-p-coumaroylquinic acid, resulted in lower free-binding energy scores when compared with two of the commercially available gliptin inhibitors. The results revealed that bioactive compounds in G. bicolor are potential natural inhibitors of DPPIV.

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Vanname shrimp is an important economic commodity that has carapace waste which is rich in the pigment astaxanthin. This study aims to determine (1) the chemical composition (proximate) of vanname shrimp carapace flour (2) the astaxanthin content of vanname shrimp carapace flour with different extraction times (3) Randemen of astaxanthin extract of vanname shrimp carapace flour. This research consisted of two stages, namely (1) Preparation and analysis of the chemical composition of vanname shrimp carapace flour (2) Extraction and analysis of the astaxanthin content of vanname shrimp carapace with different extraction times. Experimental research method with completely randomized design (CRD). The different extraction time treatments consisted of three tare, namely 120, 180, and 240 minutes. The data obtained were analyzed using Analysis of Variance (ANOVA). The results showed that carapace flour had a chemical composition of moisture content of 10.28%, ash content of 33.73% db, protein content of 38.82%db, fat content of 1.56%db, crude fiber of 25.9%db. Meanwhile, the highest astaxanthin content from this study was 5.0909 mg / g (240 minutes). the resulting levels were 2.6351 mg / g (120 minutes), 3.2569 mg / g (180 minutes) and 5.0909 mg / g (240 minutes), respectively. The yield of astaxanthin extract produced were 50.95% (120 minutes), 65.36% (180 minutes), and 78.92% (240 minutes), respectively. The highest yield of astaxanthin extract in the study was in the 240 minute treatment of 78.92%.

Various phenolic and flavonoid compounds that are secondary plant metabolites are known to contribute to physiological wellbeing. Extraction efficiency of such compounds from plant sources is dependent on the extraction solvent type and composition, and its pH. In this study, different extraction variables were examined: heating time (20 to 180 min), temperature (60˚C, 75˚C and 90˚C) and pH (2.5, 3.0, 4.0, 5.0, 6.0 and 7.0). Hot water was used in the extraction of dry samples. For phenolics, the most efficient extraction was by using water at 60˚C for 180 min, whereby 5.95 mg GA equivalent/dry extract was achieved. The most efficient extraction of flavonoids was achieved with water at 60˚C for 150 min, whereby 43 µg Quercetin equivalent/dry extract was obtained. Adjusting the solvent to pH 2.5 increased the yield to 45.3 µg Quercetin equivalent/dry extract.