Morphological traits and two kinds of molecular markers were employed to study the genetic relationships among improved rice (Oryza sativa) varieties of Indonesia since 1943. Dendrograms based on morphological traits and both molecular markers (simple sequence repeats, SSR and single nucleotide polymorphism, SNP) agreed in separating the varieties into two primary groups. Based on the morphological traits, a larger group ([60 %) contains varieties with smaller sizes compared with those in the smaller group (\40 %). SSR and SNP markers revealed that most of the varieties belonged to indica (88; 89 %) and japonica (9; 8 %) subspecies, and 3 % of varieties were not involved in two subspecies. The molecular markers revealed that the genetic diversity (H) stagnated between stage II (1967)(1968)(1969)(1970)(1971)(1972)(1973)(1974)(1975)(1976)(1977)(1978)(1979)(1980)(1981)(1982)(1983)(1984)(1985) and stage III (1986)(1987)(1988)(1989)(1990)(1991)(1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999)(2000)(2001)(2002)(2003). However, during stage I , H was higher than in the other stages as revealed by SNP markers, while H in stage I was lower than in the other stages as revealed by SSR markers. In this study, the two molecular data sets were positively correlated and positive correlations between the phenotypic and molecular data depended on the kind of molecular marker: SNP had higher Mantel r values than SSRs. Besides, SSR markers seem to be appropriate for pedigree studies, while SNP markers could be used to reveal genomic relationships. These findings were attributable to the different properties of these two different markers. These results suggested that the diversity and differentiation of both the phenotypic and molecular marker variations were probably resulted from the crossing and selection in rice breeding in Indonesia. We suggest that Indonesia needs another strategy to improve new varieties to avoid a reduction in genetic diversity and similarity.

Abstract. Seed storage is a post-harvest activity that is done to maintain the seed quality before planting. The problem often encountered in seed storage is the rapid reduction of seed quality in short period of time. Seed storability is important to maintain seed quality in good condition. The aim of this research was to find out the best seed storability of 16 genotypes of UNPAD Hybrid Maize Seeds after 4 months storage period. This research was done by identificating best seed storability after some storage period of single hybrid maize seed, namely, DR (Downey Resistance), BR, MDR (mutant of DR), and MBR (mutant of BR) which are a collection of Maize Development Team Plant Breedung Laboratory Faculty of Agri-culture Universitas Padjadjaran. This research was conducted from October 2016 until February 2017 at Seed Technology Laboratory Faculty of Agriculture Universitas Padjadjaran. Completely Randomized Design was used and repeated two times. Data were tabulated and analyzed using the F test, while to test the significant difference further were using Scott Knott test level of 5%. The result showed that there is significant difference in electrical conductivity value, 1000 grain weight, seed germination capacity, vigor index, seed growth simultaneously, and normal seedling dry weight after 4 months storage period. MDR 18.5.1, DR 17, 4BR 157, 4MDR 14.1.1  were the best genotype on seed storability after 4 months storage period.Keywords : maize, genotypes, seed storability,  storage period Sari. Penyimpanan benih merupakan kegiatan pascapanen yang dilakukan untuk memper-tahankan mutu benih hingga benih tersebut siap ditanam. Permasalahan yang sering dihadapi pada penyimpanan benih yaitu penurunan mutu benih secara cepat dalam periode yang belum terlalu lama. Tujuan dari penelitian ini adalah untuk mengetahui ketahanan simpan 16 genotip benih jagung hibrida UNPAD yang terbaik setelah periode simpan empat bulan. Penelitian ini dilakukan dengan mengiden-tifikasi ketahanan simpan terbaik setelah bebe-rapa periode simpan dari genotip benih jagung hibrida tunggal, yaitu genotip DR (Downey Resistance), BR, MDR (mutan DR), dan MBR (mutan BR) yang merupakan koleksi Tim Pengembangan Jagung Laboratorium Pemuliaan Tanaman Fakultas Pertanian Universitas Padja-djaran. Penelitian dilakukan pada bulan Oktober 2016 sampai Februari 2017 di Labora-torium Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Penelitian ini dilakukan dengan menggunakan Rancangan Acak Leng-kap (RAL) 2 ulangan. Data dianalisis mengguna-kan uji F, sedangkan perbedaan yang signifikan antar perlakuan digunakan uji Scott Knott pada taraf nyata 5%. Hasil penelitian menunjukkan terda-pat perbedaan yang signifikan pada parameter daya hantar listrik, bobot 100 butir, daya berkecambah, indeks vigor, keserempakan tum-buh, serta bobot kering kecambah normal setelah periode simpan 4 bulan. Genotip MDR 18.5.1, DR 17, 4BR 157, 4MDR 14.1.1 merupakan genotip yang memiliki ketahanan simpan  setelah periode simpan 4 bulan.Kata kunci: jagung, genotip, ketahanan simpan, periode simpan

Background: Kelabau (Osteochilus spp.) is a freshwater fish commonly found in the rivers of Riau, Indonesia. Researchers believe that these are Osteochilus kelabau; however, accurate taxonomic determination of these fish in Riau waters has not been made. The purpose of this study was to facilitate the identification of the kelabau based on its morphology and genetics using biometric and cytochrome c oxidase subunit 1 (CO1) analyses, respectively. Methods: Fish samples were collected from the Siak, Kampar and Rokan rivers in Riau Province, Indonesia. The DNA of 90 fish was extracted from the caudal fins using a DNA extraction kit, after which it was amplified using primers Fish-F1 and Fish-R1. Sequencing was conducted by Applied Biosystems Macrogen Korea, and the DNA sequences were then edited and aligned using MEGA v. 7. All samples were BLAST-searched for identification using the National Center for Biotechnology Information and BOLD System. Phylogenetic trees were constructed, and the similarity index was calculated using accession numbers AP011385.1 and KC631202.1 in GenBank. Results: Analysis of the consensus barcode sequence for 86 species revealed a high percentage of barcode matches (96%–97% in GenBank and 96.6%–96.76% in the BOLD System). The nucleotide distance between groups of kelabau from the different rivers based on the Kimura 2-parameter model gave the following results: 0.05% between groups from the Siak and Kampar rivers, 0.09% between those from the Siak and Rokan rivers and 0.05% between those from the Kampar and Rokan rivers. The nucleotide distance between the groups in the Siak (0.09%), Kampar (0.00%) and Rokan (0.10%) Rivers indicated that the kelabau in those rivers were related to each other. Conclusions: Based on the results of the research data using CO1 and biometric analyses, the kelabau were confirmed to be O. melanopleurus.

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