One factor affecting productivity is the quality of seeds with good initial seed vigor indicators, but less than optimum growing environment conditions also affect seeds' growth and initial Vigor. One of the technologies that can improve it is giving priming treatment to the seeds before planting. This study aims to see the effect of priming treatment to improve the quality of Vigor and early growth of 2 composite corn varieties, namely Sukmaraga and Bisma. The research was carried out in the laboratory and the Green House, with the experimental method using a Completely Randomized Design (CRD) with each seed of the two varieties treated with Hydropriming (soaking seeds before planting) using Ion-Free Water (IFW) for 30 minutes, 12 hours, and 24 hours plus a control treatment without Priming, and priming using PGR made from sodium orthonitrifenol 0.2%, sodium 2,4-dinitrophenol 0.05% both as controls. So there are a total of 10 treatments. The results showed that priming treatment greatly influenced the germination, where all Hydropriming treatments gave average germination above 88%, significantly different from seeds without priming below 82%. The hypocotyl length ratio of Sukmaraga and Bisma corn seeds was also different from the treatment without priming. Seeds with hydropriming for 12 hours showed the best field growth percentage, reaching 96.7% (Sukmaraga variety) and 96.98% (Bisma variety).
Cancer is a large group of diseases that occur in almost any organ or tissue of the body when abnormal cells get out of normal and invade adjacent cells. In women especially, breast cancer is among the most common types of cancer. These cancer cells will form a tumor that can be seen on an x-ray. Some traditional breast cancer treatments have been carried out, including using local herbal plants such as rodent tubers (Typhonium flagelliforme). Rodent tuber contains stigmasterol which has a high anticancer effect and was found in several mutant clones. The purpose of this study was to analyze DNA differences between mutant and wild-type rodent tuber plants based on specific RAPD markers and sequence differences in each sample. The RAPD OPB18 primer was successfully amplifies polymorphic DNA bands with sizes ranging from 700 to 1000 bp. As a result of the sequencing analysis, separate segments in wild-type and mutant plants had different sequences. In comparing the test sequence to the NCBI database, it was found that wild-type rodent tuber samples matched Gossypium raimondii (81.82%) and BLAC (bulk highest anticancer compound) rodent tuber mutant plants has similarity with Slanum pinnasectum about 87.36%. Based on this results, the similarity of rodent tuber sequences is possible to be developed in the specific sequences that encode a gene, especially genes encoding anticancer compounds.
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