There are many natural remedies derived from herbs have been used as alternatives to treat kidney stones disease effectively including Euphorbia hirta L. and Orthosiphon stamineus. Therefore, the present study aims to investigate the effect of four different extraction solvents of E. hirta and O. stamineus including hexane, methanol, ethyl acetate and water to inhibit the crystallization of calcium oxalate (CaOx) in-vitro. The inhibition of crystallization was studied by using rate of CaOx aggregation assay and was determined by UV-Vis spectrophotometer. Cystone was used as positive control. The result showed the best inhibition in aggregation of CaOx was exhibited by hexane extract of E. hirta (95.78%) which had no significant difference with Cystone, and the lowest inhibition was shown by hexane extract of O. stamineus (20% ±). It could be related to the presence of higher amount of alkaloid in hexane extract of E. hirta as compared to other extracts. In conclusion, both of plant extracts showed antiurolithiatic properties by inhibiting the crystallization of calcium oxalate. However, the rate of inhibition for both plants varies depending on the type of solvent used.

The Phyllanthus niruri is traditionally used for curing of kidney disorders and urinary stones in Malaysia. Hence the current work was aimed to evaluate the effect of different solvents extract (n- hexane, ethyl acetate, methanol and water) of P. niruri for in vitro anti-urolithiatic properties in terms of inhibition activity on CaOx by using the rate of CaOx aggregation assay and dissolution of calcium oxalate (CaOx) crystal by using titrimetry method. Cystone was used as positive control. The effects of cystone on slope of nucleation and aggregation as well as growth of CaOx were evaluated spectrophotometrically. The highest yield percentage of P.niruri was occupied by methanol (5.74 %). The maximum inhibition against aggregation of CaOx crystals was also occupied by methanol (66.67 % ± 1.61) and was comprised with alkaloid, steroid, terpenoid and tannin. Dissolution effect on calcium oxalate crystals indicates that the aqueous extracts of P. niruri was found to be more effective in dissolution of CaOx with 63.33 %   ± 1.44. P. niruri significantly (P < 0.05) inhibited the slope of nucleation and aggregation of CaOx crystallization, and reduced the crystal density. The results of the present study confirmed that P. niruri leaves can be used as remedial mediator for urolithiasis. However, further studies are required for isolation and identification of active constituents and their in-vivo confirmation.

Background. Ethnobotanical studies report the customary uses of plants used by the local communities across the world. The goal of present study was to census the ethno-medicinal uses of local plants used by the natives of Tehsil Kallar Syedan, District Rawalpindi by using quantitative ethnobotanical indices.Methodology. Ethnobotanical surveys were carried out during March 2018 to April, 2022 to document traditional uses of medicinal plants by using semi-structured questionnaires. The gathered data was examined by using quantitative indices comprising frequency of citation (FC), relative frequency citation (RFC), use value index (UV) and fidelity level (FL%).Results. In total, 169 medicinal plant species belonged to 136 genera and 54 families have been documented from this region which are being used to treat or prevent common diseases. The informants included people of different age groups. Poaceae was the most widely used family (17 species). Major life form of plants was herb (61%); while leaves (35%) were highly used in preparing recipes. RFC values ranged from 0.11 to 0.78; while UV ranged from 0.10 to 0.82. A total of 13 species showed highest FL of 100%. Most medicinal applications involved the whole plant (50.8%), although leaves (32.5%), roots (10.0%), flowers (8.2%), stems (5.9%), seeds (3.5%), bark (3.5%), fruit (1.7%), latex (1.7%), bulbs (1.1%), rhizomes (0.5%), and tubers (0.5%) were also used.Conclusion. This research appraisal provides some additional and novel use along with higher RFCs and UVs which may serve as benchmark for phytochemical analysis and novel therapeutic properties.

Ehretia acuminata, commonly known as “Puna” in Pakistan, is from the Boraginaceae family and is used in ecological, medicinal, and agricultural sectors. The current study was conducted to assess the pharmacognostic potency of bark extracts from E. acuminata. The crude distilled water, ethanol, and chloroform extracts signified a concentration-dependent increase in intestinal mobility of the experimented animal, and the plant delivered methodical proof for its pharmacological usage as an antispasmodic drug. The bark distilled water, bark ethanol, and bark chloroform extracts revealed (11±1, 9±1, and 11±1) antispasmodic potential at (300 mg/kg). The distilled water, ethanol, and chloroform extracts also showed analgesic potential in the present study and the results were concentration dependent. The bark distilled water, bark ethanol, and bark chloroform extracts revealed the (10±1,16±1, and 11±1) analgesic potential at (300 mg/kg). The distilled water, ethanol, and chloroform extracts also showed the muscle relaxant potential in this study and the results were concentration dependent. The Bark distilled water, Bark ethanol, and Bark chloroform extracts revealed the (6±1, 5±1, and 5±1) at (300 mg/kg). While the distilled water, ethanol and chloroform extracts did not show acute toxic effects against the tested animal mice. In this study, bark extracts of E. acuminata showed pharmacological potency in experimental animals. The plant delivered scientific proof for its pharmacological usage as an antispasmodic, acutely toxic, muscle relaxant, as well as an analgesic drug.