Several bacteria were isolated from straw mushroom (Volvariella volvacea) cultivation medium. There are three potential isolates previously characterized and has growth inhibition effect against V. volvacea. This screening result lead to the further study about the inhibition activity against phytopathogenic fungi. The aim of this research is to investigate the antifungal activity of three bacterial isolates against three phytopathogenic fungi and identification of the bacteria. The method used in this study are antifungal assay using co-culture method and disk difussion assay using the filtrate of each bacteria. The profile of antifungal compound was identified using ethyl acetate extract followed by evaporation and gas chromatography (GC-MS) analysis. Identification of each isolates was performed using 16S rDNA amplification and sequencing. Three phytopathogenic fungi i.e Cercospora lactucae (InaCC F168), Colletotrichum gloeosporides (InaCC F304) and Fusarium oxysporum f.sp. cubense (F817) were co-cultured with bacterial isolates C2.2, C3.8, and D3.3. The C3.8 isolate has highest average inhibition activity either using isolate and filtrate. The result relatively consistent against three phytopathogenic fungi. The metabolite profile of C3.8 isolate showed the Bis(2-ethylhexyl) phthalate as the main compound with 97% similarity. Bis(2-ethylhexyl) phthalate has potential effect as antibacterial and antifungal compound. According to EzBioCloud and GeneBank databases, the C2.2 isolate identified as Bacillus tequilensis, C3.8 as Bacillus siamensis and D3.3 as Bacillus subtilis subsp. Subtilis. This study also shows the potential of Bacillus siamensis C3.8 as biocontrol against phytopathogenic fungi.

This research aims to identify student learning motivation in online practicum courses during the COVID-19 pandemic. This study used a quantitative survey method toward 117 biology students at the Walisongo State Islamic University. Data were collected using five-point Likert scale questionnaire. The data show that students learning motivation is in good criteria (75.47%). The percentage breakdown and criteria for each indicator are as follows: concentration 77.9% (good), curiosity 81.06% (very good), enthusiasm 66.67% (good), independence 82.64% (very good) ), readiness 77.78% (good), enthusiasm 66.7% (good), never give up 72.82% (good), and confidence 78.21% (good). This study's result can be used as an evaluation material for designing effective online learning during the COVID-19 pandemic.

Lerak (Sapindus rarak DC) has many benefits, including washing soap, vegetable pesticides, foaming agents for toothpaste, essential ingredients for soap gels, and antibacterial agents. Along with the many uses, it must be balanced with conservation activities to maintain the sustainability of lerak resources. This study aims to determine the usefulness of lerak (Sapindus rarak DC) and generative conservation efforts of lerak in the Jatimas community, Mijen, Semarang. This community service-based research was conducted in January-February 2021 in Jatimas, Mijen, Semarang. The research method uses the ethnobotany approach, conservation, and literature study, which is equipped with interviews and direct observations. The results show that the community uses lerak as herbal soap, jewelry washing agent, and insecticide. Through this community service program, the community has carried out generative conservation of lerak through seeds.