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Ponceau 4R(E124) is azo dye that is mostly used in some of food products such as soft drinks, beverages, jelly and ham. A simple and sensitive procedure is established for the determination of ponceau 4R using cloud point extraction (CPE). In order to get the optimum conditions for ponceau 4R extraction, various analytical factors such as pH media, nonionic surfactant TX-114 or Brij-56, cationic surfactant CPC and equilibration temperature was investigated. The linearity of calibration graph was above the range of 0.05-1.7 µg ml-1. The LOD and LOQ using TX-114 were based to be 0.025, 0.075 µg ml-1 whereas for Brij-56 were found as 0.062, 0.187 µg ml-1 respectively. The proposed procedure was successfully employed for ponceau 4R detection in several beverages samples.

Benzidines and phenols are the most priority pollutants. Separation and quantitative estimation of priority pollutant benzidines composed of various benzidines BZ, including substituted 3, 3'-dichlorobenzidine DCB and 3, 3'-dimethylbenzidine DMB, and priority pollutant phenols (9 compounds, i.e., phenol, 2-and 4-nitrophenol, 2,4-dimethylphenol, 2-, 2,4-di-, 2,4,6-tri-, Penta-chlorophenol, and 4-chloro-3-methylphenol)was performed using high performance liquid chromatography-ultra violet techniques. Both groups were separated using a C-18 column with a UV detector at a wavelength of 280 nm, and the flow of the mobile phase was isocratic. The mobile phase consisted of 75:25 methanol: water. The column temperature was 50°C, and the flow rate was 1.8 ml/min for the Benzedine's separation. The mobile phase consisted of a 50:50 acetonitrile: phosphate buffer. The optimum pH was 7.1, the flow rate was 0.7 ml/min and the optimum column temperature was 45°C for the phenols separation. The separation parameters were calculated, including the chromatographic parameters such as the capacity factor (k), the number of theoretical plates (N) , the selectivity factor (α), and the resolution factors (Rs).This method was applied to real samples. The water samples that were analyzed were obtained from a petroleum refinery wastewater treatment unit. The results ranged between undetectable levels and 246.9μg/L of the selected benzidines.The results were ranged between undetectable levels and 1865.61 μg/L of the selected phenols.

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In this paper sixteen Polycyclic Aromatic Hydrocarbons (PAHs) are estimated in sediments in five locations along Diyala River where entering Baghdad city until flows into Tigris River, (PAHs) are organic compounds that contain two or more fused rings. Some of these compounds are classified as carcinogenic and mutagenic pollutants. Soxhlet technique is used in this work to extract PAHs from sediment samples with using methylene chloride as extraction solvent. High Performance Liquid Chromatography (HPLC) with UV-Vis detector is used for determination of PAHs. According to the results the maximum of total PAHs concentration in the sediment are found in the location No. 4 with1211.22mg/kg in summer and 211312 mg/kg in winter, while the minimum of total PAHs concentration are found in