Spectrophotometeric Determination of Ponceau 4R in the Several Beverages Using Mixed Micelles after Cloud Point Extraction

Abstract

Ponceau 4R(E124) is azo dye that is mostly used in some of food products such as soft drinks, beverages, jelly and ham. A simple and sensitive procedure is established for the determination of ponceau 4R using cloud point extraction (CPE). In order to get the optimum conditions for ponceau 4R extraction, various analytical factors such as pH media, nonionic surfactant TX-114 or Brij-56, cationic surfactant CPC and equilibration temperature was investigated. The linearity of calibration graph was above the range of 0.05-1.7 µg ml-1. The LOD and LOQ using TX-114 were based to be 0.025, 0.075 µg ml-1 whereas for Brij-56 were found as 0.062, 0.187 µg ml-1 respectively. The proposed procedure was successfully employed for ponceau 4R detection in several beverages samples.

Synthesis of New Azo Compound and its Application for Spectrophotometric Determination of Sulfamethoxazole and Extraction using Cloud Point Extraction

“…[14][15][16][17] Cloud point extraction has grown in popularity in comparison to other extraction techniques due to the advantages of low organic solvent consumption, fast recovery, low cost, high enrichment factor, and rapid phase separation.It is appealing because it reduces the employing and exposures to the solvent, as well as the time and costs of extraction and removal that have been used for pre-concentration of sulfomethoxazole after the formation of an azo compound that is poorly H 2 O-soluble. [18][19][20][21][22][23][24] The aim of this work, is synthesis of azo derivative of sulfomethoxazole with salbutamol using azo coupling reaction and use this reaction for determination of sulfomethoxazole spectrophotometrically, then extraction with cloud point extraction technique.…”

Determination of Sulfacetamide Sodium in Pure and Their Pharmaceutical Formulations by Using Cloud Point Extraction Method

“…spectrofluorimetric (13,14), TLC (15), enthalpimmetric (13) and HPLC with fluorescence detection (15). The cloud point extraction has a great importance due to safety , speed and low cost , consequently it has applied as one of the evaluation and pre-concentration techniques in analytical chemistry (16)(17)(18)(19)(20). In this work, the proposed technique is based totally on the azo coupling reaction of SAC with para-cresol to form an orange solution, then on the estimation and preconcentration the usage cloud point extraction (CPE) which suggests an absorbance at 450 nm.…”