Carbon monoxide (CO) is proposed as an active pharmaceutical agent with promising pharmaceutical prospects, as it has been involved in multifaceted modulation of diverse physiological and pathological processes. However, questions remain for therapeutic application of inhaled CO attributed to the inherent great affinity between CO and hemoglobin. Therefore, a robust platform with the function of CO transport and controllable release, depending on the local status of an organism, is of prominent significance for effectively avoiding the side effects of CO inhalation and optimizing the biological regulation function of CO. Utilizing the oxidative stress biomarker H O as a trigger and combining with photo-control, a two-photon H O -activated CO photoreleaser, FB, featuring highly sensitive and specific H O sensing and photocontrollable CO release, was developed and the vasodilation effect of CO against angiotensin II was demonstrated.

Drug-induced liver injury (DILI) has aroused wide concern. Finding new markers or indicators as well as detoxification molecules for DILI is of great significance and good application prospect, which can help develop effective preclinical screening methodology and corresponding treatment protocols. Herein, in this article, DILI caused by antidepressant drugs of duloxetine and fluoxetine and its remission were evaluated by a two-photon fluorescent probe, RPC-1, through discriminating and imaging HClO and HS simultaneously. By being applied both in vitro and in vivo, RPC-1 revealed slight up-regulation of HClO and negligible liver damage after administration of either of the two drugs. In contrast, an apparent up-regulation of HClO and obvious liver damage was observed after combined administration of the drugs. Meanwhile, the pretreatment of N-acetyl cysteine (NAC) resulted in the increasing of endogenous HS level, which contributed to the remission of DILI. The histological analysis and serological test both gave good consistency with the imaging results. These findings demonstrate that HClO may be an appropriate indicator of DILI, and HS plays an important role in the antidotal effect of NAC. We envision that RPC-1 can be used as a powerful tool to predict clinical DILI and study the effect of antidote, as well as explore the molecular mechanisms involved.

We report for the first time the development of a two-photon excitable NO photoreleaser, CNNO, for ratiometric imaging and tracking of NO release in live cells. CNNO exhibits the merits of spatiotemporal control in both the site-specific NO release in the selected cell culture region and the controllable vasodilation of mouse aorta ex vivo.

A tetraphenyl ethylene (TPE)-based fluorescent probe specific for ONOO− was developed to evaluate and verify the protective effect of estrogen on myocardial cells during OGD/R.

Carbon monoxide (CO) is proposed as an active pharmaceutical agent with promising pharmaceutical prospects, as it has been involved in multifaceted modulation of diverse physiological and pathological processes. However, questions remain for therapeutic application of inhaled CO attributed to the inherent great affinity between CO and hemoglobin. Therefore, a robust platform with the function of CO transport and controllable release, depending on the local status of an organism, is of prominent significance for effectively avoiding the side effects of CO inhalation and optimizing the biological regulation function of CO. Utilizing the oxidative stress biomarker H2O2 as a trigger and combining with photo‐control, a two‐photon H2O2‐activated CO photoreleaser, FB, featuring highly sensitive and specific H2O2 sensing and photocontrollable CO release, was developed and the vasodilation effect of CO against angiotensin II was demonstrated.