No abstract

Polycarbonate and polypropylene microchips were made by thermoforming and sealed by special PCR films. The aim of this work was to study the possibility of promptly manufacturing microchips for performing RT-PCR analysis. Microchips are made of hard plastics, preferably using domestically produced materials. Therefore, to achieve the goal, the following tasks were set: 1) thermoforming the topology of the chambers in polycarbonate and polypropylene master mold made by laser microforming; 2) sealing the obtained microchips with commercial films for PCR, which are polymer films with a thin adhesive layer (the connection should provide a binding force, holding the pressure arising in the chambers during thermal cycling, and not inhibiting the reaction); 3) study of light transmission and background fluorescence of microchips, since it is supposed to detect PCR-RT by optical methods; 4) study of the properties of the surface of the chambers of microchips (by the recumbent drop method), since they affect the formation of air bubbles during thermal cycling; 5) approbation of microchips when performing PCR-RT in them. The efficiency of microchips was demonstrated by conducting PCR-RT on a test object. The obtaining microchips made of polycarbonate and polypropylene could withstand storage at negative temperatures (–40 ℃).

The dominant trend in molecular genetic methods research of biological samples is the automation of all stages, the most valuable of which is preparation of the sample. The use of microfluidic technologies provides not only full control of all operations at this stage, but also reduces the impact of the environment on the sample preparation process. Magnetic beads technology is one of the methods for extracting RNA and DNA with subsequent analysis. The paper presents magnetic beads-based nucleic acids extraction in the microfluidic chip via automatic mode with further registration by real-time polymerase chain reaction. We have shown that efficiency of DNA extraction in microchip is compared to that in case of conventional manual extraction and allows further improvement by means of automation.

No abstract

No abstract