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We developed a technique for fabrication microfluidic silicon-glass chips with a system of nanochannels connecting two microchannel using traditional optical lithography and a focused ion beam. To investigate the transport phenomena in the nanochannels we experimentally studied their ion conductivity and using optical microscopy confirmed the existence of the diffusion flow through them. The developed method allows us to create systems of nanochannels with on-purpose geometry and controlled sizes. Devices with such nanochannels can be applied in the creation of biosensor devices and for genetic studies.

The dominant trend in molecular genetic methods research of biological samples is the automation of all stages, the most valuable of which is preparation of the sample. The use of microfluidic technologies provides not only full control of all operations at this stage, but also reduces the impact of the environment on the sample preparation process. Magnetic beads technology is one of the methods for extracting RNA and DNA with subsequent analysis. The paper presents magnetic beads-based nucleic acids extraction in the microfluidic chip via automatic mode with further registration by real-time polymerase chain reaction. We have shown that efficiency of DNA extraction in microchip is compared to that in case of conventional manual extraction and allows further improvement by means of automation.

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