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Two strains of Salmonella typhimurum LT2, SA1475 and MA411, were fortuitously found to be sensitive to bacteriophage Mu. The Mu-sensitivity allele of SA1475 was called musAl and shown to be linked to the histidine operon both in conjugation and transduction experiments. The Mu8 allele of MA411 was unlinked to the his region and was tentatively designated musB2. Strains carrying large deletions of the his operon were also tested for Mu sensitivity; those in which the his-rfb region is deleted were also sensitive to Mu. Transduction data led to the order zee-2 hisOGDCBAHFIE gnd musA. An Hfr injecting the his operon early (HfrK9) and carrying hisG9424::TnlO A4 All and musAl was isolated; this Hfr made it possible to introduce the Mu' character into most derivatives of S. typhimurium LT2. Since strain SA1475 is resistant to bacteriophage P1, it could be used to select a new Pl-Mu hybrid which has the host range of Mu and the transduction properties of P1.

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