An experiment was conducted at the tissue culture laboratory of MGM college of Agricultural Biotechnology, Aurangabad (M.S.) during 2013-14 to evaluate the effects of different concentration levels of auxins viz., NAA and IAA on growth and quality of cotton (Gossipium hirsutum L.) ovules under invitro conditions. The experiment was laid out in Completely Randomized Design, 7 different concentration levels of auxins IAA and NAA. Auxins were tried at the levels of 0, 5, 10 and 15 µM conc. of each in culture of 2 DPA flowers of G. hirsutum L using Beasley and Ting (BT) medium. Cultures were maintained for 21 days and fibre length, fibre weight, ovule length and ovule weight were recorded. Different concentrations of auxins significantly influenced development of cotton ovules under in-vitro conditions. NAA at concentration of 15 µM was found significantly superior over rest of the levels of IAA and NAA in case of fibre length, ovule length and ovule weight whereas IAA concentration of 15 µM was found significantly superior over rest of other concentrations of IAA and NAA for increasing fibre weight.

Momordica cymbalaria is a tuberous and monoecious species. It is rare and endemic medicinal plant found to be distributed at few localities in Maharashtra, A.P. and Karnataka. Due to its food and medicinal value the species is extensively exploited by the local people hence it is the time to protect the species by means of conservation. Leaf is a photosynthetic part of the plant body. There are relatively very few reports about leaf as an explant in cucurbits. So In the present study In vitro culture technique is used as a conservation strategy for M. cymbalaria as an explant.

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