This study investigated anticancer, anti HIV-1, antimicrobial potentials and the phytochemical composition of the total methanol extract, petroleum ether (60-80°C) and dichloromethane fractions of Citrus volkameriana leaves. Anticancer activity was measured by a sulforhodamine B (SRB) assay. Syncytia formation assay was used to evaluate anti-HIV-1 potential and antimicrobial assay was performed according to Buwa and Van staden with some modifications. The results showed that petroleum ether fraction exerted growth inhibitory effect on melanoma cancer cell line (UACC62), it inhibited the syncytia formation of HIV-1 with EC 50 of 17.4 µg mLG
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