The major disadvantage in cervical vertebral maturation stages (CVMS) is the involvement of radiographic exposure. This study was undertaken to assess the applicability of insulin-like growth factor I (IGF-I) blood level as a maturation indicator by correlating it to the CVMS. METHODOLOGY: This cross-sectional study was conducted at orthodontic department of our institute. With 80% power of study, 5% desired level of significance and using 0.67 correlation value, a sample size of 75 was calculated. There are five stages of CVMS and in each CVM stage 15 subjects were allocated, therefore collective sample size was 75. The technique of sampling was purposive (non-probability) sampling. Out of 75 patients, 47 (62.7%) were males and 28 (37.3%) were females. The mean ages of the patients were 12.5 ± 2.6 years. Analysis of variance (ANOVA) test was performed to evaluate the blood serum IGF-1 levels among five stages of CVMS. RESULTS: There was a statistically considerable difference in mean IGF-1 among five stages of CVM. The mean IGF-1 (ng/ml) of CVMS 1 was 204.

Objectives: To determine prevalence of deep bite and gender association with respect to age. Study Design: Cross-Sectional study. Setting: Department of Orthodontics Nishtar Institute of Dentistry, Multan. Period: 6 months i.e. from 15 December 2018 to 14 June 2019. Material & Methods: A study with 200 (126 females, 74 males) patients were taken as a sample after being examined in Chi square test was applied to determine association. Results: Deep bite was observed in 43% of patients in total sample of 200. Out of which 61.1% is found in females and 38.4% in males. It is also observed that age is an effect modifier with respect to gender. Conclusions: There as an insignificant difference in distribution of deep bite males and females. It is observed that with increasing ages deep bite decreases considerably.

Background: The individual effects of nicotine and caffeine have been reported in previous studies but their combined effect on tooth movement needs to be elucidated. The objective of this study was to evaluate the combined effect of nicotine and caffeine on the magnitude of orthodontic tooth movement (OTM) in rats. Material and Methods: This experimental study was conducted on Sprague-Dawley rats (Animal House and Pathology Laboratory; Post Graduate Medical Institute, Lahore) in the department of Orthodontics, de’Montmorency College of Dentistry, Lahore from 8th July 2014 to 8th January 2015. Forty male Sprague-Dawley rats were divided into four equal groups: Control group (CR), nicotine group (NT), caffeine group (CF) and combined nicotine and caffeine group (CNC). Closed coil nickel titanium (NiTi) spring was placed between incisor and maxillary molar. Nicotine group (NT) was treated by intraperitoneal injections of nicotine. Caffeine was given to caffeine group and Combined nicotine and caffeine group (CNC) was treated in the same way as individual nicotine and caffeine groups daily for 14 days. All the rats were sacrificed on 15th day. Magnitude of the orthodontic tooth movement was measured using digital Vernier caliper. Means and standard deviation were calculated for orthodontic tooth movement. One-way ANOVA was used to determine the mean difference in OTM. Post hoc Tukey test was used for multiple comparisons among the groups. Results: The mean orthodontic tooth movement (OTM) was 0.32 mm ± 0.05 in control group, 0.56 mm ± 0.04 in nicotine group, 0.52 mm ± 0.034 in caffeine group and 0.8 mm ± 0.06 in combined NC group, respectively. The difference between mean OTM among the groups was statistically significant (P-value <0.001). The mean OTM in CNC group was significantly higher as compared to other groups (CR, NT, CF, NT) (P-value <0.001). Conclusions: In rats, the combined use of nicotine and caffeine results in greater orthodontic tooth movement as compared to their individual use.