No abstract

In this study, ion pair reactions used for evaluation of phenylephrine HCl the development of simple, accurate, low-cost, and fast extraction methods for the separation of phenylephrine HCl was described. The first method was used was the direct extraction that included the reaction PHE with Alizarin yellow reagent to produce yellow complex in the acidic medium (pH=4). The absorbance of this complex was 430nm. The concentration range (1-20µg.mL-1), the Beers law was obeyed with correlation coefficient (R2=0.997), limit of detection as (0.34µg.mL-1), limit of quantification as (1.12µg.mL-1) and molar absorptivity as (14459.9). The second method was dispersive liquid liquid microextraction (DLLME). This method was used to estimation PHE complex by utilizing UV-Vis spectrophotometer. The linearity of calibration curve above was the range between (0.5-13 µg.mL-1), the correlation coefficient (R2=0.996) and molar absorptivity was (12423.3), the detection limit (LOD) and quantification limit (LOQ) were (0.094µg.mL-1) and (0.31µg.mL-1), respectively. This process was successfully method to detect PHE in both pure and pharmaceutical formulations.

In this study, ion-pair reactions were used to investigate bromohexine HCl. The development of simple, accurate, sensitive, low-cost, and efficient extraction methods for bromohexine HCl separation, such as the DLLME and cloud point extraction techniques, are described as bromohexine HCl estimation methodologies. These methods employed the interaction of bromohexine HCl with alizarin yellow reagent to produce a yellow complex in an acidic medium (pH = 5). The complex's maximum absorbance intensity was 480 nm, and the stoichiometry for both continuous variation and molar ratio methods was determined to be 1:1. The dispersive liquid liquid microextraction (DLLME) method's concentration range (1-23µg.mL -1 ), the Beers law was obeyed with correlation coefficient (R 2 =0.998), limit of detection as (0.055µg.mL -1 ), limit of quantification as (0.183µg.mL -1 ) and molar absorptivity as (23930.2L.mol -1 .cm -1 ).In the second technique, the cloud pointextraction method, The linearity of calibration curve above was the range between (1-40 µg.mL -1 ), the correlation coefficient (R 2 =0.998) and molar absorptivity was (13202.88L.mol -1 .cm -1 ), (LOD) and (LOQ) were (0.141µg.mL -1 ) and (1.4641µg.mL -1 ), respectively. The proposed techniques can be used for the determination of bromohexine HCl in both pure and pharmaceutical formulations with very good success.

Aim and Objective: Tomato juice and paste have been used successfully in removing human skin corn, because of the presence of organic acids in them (tomato juice and paste of green and red ones). These acids are efficient to destruct and dissolve the skin corns. The juice and paste are attempted firstly on the sheepskin and meat; consequently, they are found efficient and active in dissolving the skin and meat proteins. Since some of human skin corns are pressed and become semisolid, this case requires periodic treatment. The objective of this paper is to attempt using of tomato juice and paste of both red and green ones in removing warts of the human body. It starts from the selection of the efficient juice in dissolving the sheepskin and meat and then testing them on human warts. Materials and Methods: Fresh tomatoes juice was prepared from 20 to 50 g of tomatoes; total acid content measured by both potentiometric and manual titrations methods. Citric acid crystals were identified by Fourier-transform infrared spectroscopy. Effect of dissolving and destruction of warts by tomatoes juice and paste was studied on sheepskin. Result and Discussion: Clear equivalence point indicates high levels of organic acids in tomatoes (green and red) showed a similar action to salicylic acid which encouraged the use of tomatoes' juice and paste for human corns for the removal of human corns. Green tomato juice demonstrated 53.20% corn dissolving and destruction in sheepskin, while with sheep meat, it is 79.75%. Red-ripened tomato juice shows only 2% less activity as compared to green tomato juice due to lycopene and less acid content. Conclusion: The research confirms high efficiency of tomato juice and paste in removing human corns without side effects or pains during the period of treatment in the sheepskin.