: Lack of sleep generates many disorders; bruxism is one of them. It has affected almost 31% of the world population. The purpose of this paper is to determine the volume of the research conducted on bruxism and to create a database. We highlight critical issues for further research commitments and communications. This paper designs a comprehensive and very perception-based picture of the bruxism disorder. The research based work uses three methods such as systematic mapping process, network visualization, and literature review. Software such as VOSviewer, MATLAB, and MEGA-X have been utilized to analyze the work. We have researched deep insights of information to retrieve the present understanding of bruxism disorder from dental to psychological concepts, from engineering detection to clinical treatment, and from temporomandibular disorder to biological genes. We found 10 keywords and 77 items of bruxism in PubMed, Scopus, Google Scholar and Web of Science databases based on the previous publication. These keywords and items are helpful to all type of researchers, which includes engineering, science and medical background personals. 11 genes and 75 research articles with approximately 115077 subjects for the analysis of detection, treatment, child and adolescent bruxism have been reviewed in the research work. In conclusion, it has been found that bruxism altogether has sleep, neurological, dental and genetic disorder components and is a complex phenomenon. This study has also mentioned the future direction and research gap so far conducted on bruxism and has also tried to provide goals for the upcoming research to be accomplished in a more significant and scientific manner.

Saxitoxin (STX) belongs to the family of marine biological toxins, which are major contaminants in seafood. The reference methods for STX detection are mouse bioassay and chromatographic analysis, which are time-consuming, high costs, and requirement of sophisticated operation. Therefore, the development of alternative methods for STX analysis is urgent. Electrochemical analysis is a fast, low-cost, and sensitive method for biomolecules analysis. Thus, in this study, an electrolyte-insulator-semiconductor (EIS) sensor based on aptamer-modified two-dimensional layered Ti3C2Tx nanosheets was developed for STX detection. The high surface area and rich functional groups of MXene benefited the modification of aptamer, which had specific interactions with STX. Capacitance-voltage (C-V) and constant-capacitance (ConCap) measurement results indicated that the aptasensor was able to detect STX with high sensitivity and good specificity. The detection range was 1.0 nM to 200 nM and detection limit was as low as 0.03 nM. Moreover, the aptasensor was found to have a good selectivity and two-week stability. The mussel tissue extraction test suggested the potential application of this biosensor in detecting STX in real samples. This method provides a convenient approach for low-cost, rapid, and label-free detection of marine biological toxins.

No abstract

: The fusion of secretory vesicles with the plasma membrane depends on the assembly of v-SNAREs (VAMP2/synaptobrevin2) and t-SNAREs (SNAP25/syntaxin1) into the SNARE complex. Vesicles go through several upstream steps, referred to as docking and priming, to gain fusion competence. The vesicular protein synaptotagmin-1 (Syt-1) is the principal Ca2+ sensor for fusion in several central nervous system neurons and neuroendocrine cells and part of the docking complex for secretory granules. Syt-1 binds to the acceptor complex such as synaxin1, SNAP-25 on the plasma membrane to facilitate secretory vesicle docking, and upon Ca2+-influx promotes vesicle fusion. This review assesses the role of the Syt-1 protein involved in the secretory vesicle docking, priming, and fusion.

Purpose -This paper aims to study the effect of Unethical advertising, misleading information or deception and stereotyping advertising impact on Customer purchase intention with mediating effect of word-of-mouth (WOM) in Pakistan. Based on variables, the authors tried to identify the effects of each variable to customer satisfaction. Data were collected through field research (questionnaire) among 600 Respondent who have their own different levels and fields like, Employee, students, and common people from twin city Islamabad & Rawalpindi. The results shows that unethical Advertising or misleading information and stereotyping advertising Negatively linked with customer buying behaviour or purchase intention, while word of mouth is also negatively associated with customer satisfaction. Limitations relate to the use of a non-probability sample and the restricted geographical area of the field research. There had a time constraint which could restrict many aspects of research for further elaboration.