In the Ukrainian flora such plants as Forsythia europaea Deg. et

Screening of 48 dry extracts of plants belonging to 18 species of 8 families not studied before on anticonvulsant activity, to analyze the chemical composition for the extracts with proved anti-and proconvulsant activity, and to estimate the connection of the effect of herbal products and their phytochemical composition. Methods. On the model of pentylenetetrazole-induced seizures in mice the influence of 48 dry herbal extracts with varied chemical composition has been investigated. Extracts were standardized by the content of alkaloids, flavonoids and polyphenols according to European Pharmacopoeia methods (HPTLC, HPLC, UPLC, GC-MS). Results. By the integral indicator of efficacyreduction of the mortality rateit has been found that 11 of herbal preparations have significant anticonvulsant properties, 10 extracts act as proconvulsants, and the remaining 27 extracts have practically no effect on experimental convulsions. It has been established that there is a relationship between the pharmacological effect of preparations and phytochemical composition of the plant being extracted as well as the host species (for parasitic species -Viscum album) and the degree of polarity of the extracting agents. For the extracts with expressed anti-and proconvulsant features the chemical composition has been investigated. Conclusion.The detailed analysis of relationship between phytochemical composition and influence of the extracts on the experimental seizures has found that anticonvulsant peculiarities of extracts most probably depend on rutin high content, but for the herbal medicines with proved proconvulsant properties the role of individual biologically active compounds has not been defined

The questions of standardization and quality control of both herbs and herbal remedies remain relevant, because it is well-known that product quality standards are essential, whether consumer using herbs or drugs. The necessity of the standardization methods development for the initial herbal material and capsule dosage form for the further quality control under manufacturing conditions remains relevant. Aim. The aim of our research was to develop simple, specific, accurate and reproducible methods for identification of flavonoids and phenolic acids in capsule dosage form containing Corylus avellana L. dry extract. Methods. The samples of gelatine capsules containing Corylus avellana L. dry extract for oral administration were analyzed. The analysis was carried out using Camag HPTLC system. The absorption spectroscopy determination of the sum of flavonoids was carried out using THERMO Scientific Evolution 60S Spectroscope in wavelength range of 300-600 nm. Results. As a result of HPTLC research rutine and quercitrine have been identified in capsule dosage form containing Corylus avellana L. dry extract. Among phenolic acids, neochlorogenic and chlorogenic acids have been identified. Under the given conditions, the spectrum of the test solution had a maximum absorption at wavelength 406 nm. The analysis of flavonoids total content in gelatine capsules containing Corylus avellana L. dry extract calculated as rutine has shown the content of 1,7 %. Conclusion. Effective HPTLC and absorption spectroscopy methods for determination of flavonoids and phenolic acids in capsule dosage form containing Corylus avellana L. dry extract have been developed. It has been found that described methods are promising enough for standardization of capsules with Corylus avellana L. dry extract and may be suggested for the quality control of the dosage form under manufacturing conditions