BACKGROUND: The health implications of the perceived use of electronic cigarettes (e-cigarettes) are safer than conventional cigarettes on metabolic parameters are not clearly understood. The current study evaluates the metabolic parameters as the impact of cigarette and e-cigarette exposure in high-fat-diet (HFD)-induced rats. METHODS: Twenty-four male Wistar rats were divided into four groups: i) NC: normal control group; ii) HFD Alone; iii) HFD + Cig and iv) HFD + E-Cig, administered HFD followed by cigarette or e-cigarette exposure, respectively. Six cigarettes stick with nicotine 2 mg/stick and 2 ml of e-cigarette liquid with nicotine 6 mg/ml were used for 25 cycles of exposure. In the end, the rats were sacrificed and obtained blood for metabolic parameter analysis, consisting of lipid profile, glucose, uric acid, urea, creatinine, aspartate transaminase (AST), and alanine transaminase (ALT). Statistical analysis with One-Way ANOVA with post hoc was used for high-density lipoprotein (HDL), triglyceride, total cholesterol, glucose, uric acid, urea, and creatinine. Furthermore, Kruskal-Wallis with Mann-Whitney U was used for nonparametric data such as low-density lipoprotein (LDL), AST, and ALT. RESULTS: Data of all metabolic parameters were shown a significant increase in the group of HFD Alone, HFD + Cig, and HFD + E-Cig, otherwise HDL levels. Furthermore, HFD + Cig followed by HFD + E-Cig groups were significantly higher compared to HFD Alone group. CONCLUSION: E-cigarettes were shown to be less harmful than conventional cigarettes but did not guarantee it was safe. Both cigarettes and e-cigarettes aggravated metabolic parameters in HFD-induced rats.

Conclusions The result of this study may add evidence to the microbiologic etiology of recurrent cholelithiasis and help to develop new prevention methods to avoid recurrence post EST. Conclusions This result suggests a new insight into fasting in Islam, RF, DF, and MTF can be an alternative in lowering high glucose levels caused by HFHF.

Background: Synbiotic contains antioxidant that has been suggested to improve oxidative stress induced by high-fat diet (HFD) consumption. This study aimed to evaluate the effect of synbiotic supplementation consisting of kepel (Stelechocarpus burahol) with the addition of Lactobacillus casei and L. plantarum on oxidative stress in HFD-fed rats. Methods: Twenty-five Wistar rats were divided into five groups (n=5) for eight weeks of treatment. The HFD control (HFD alone) group and three different groups supplemented with three various doses of kepel synbiotic (Syn 1.2 mL, Syn 1.8 mL, and Syn 2.4 mL) were fed HFD for the first four weeks and continued supplemented kepel synbiotic for the second four weeks. Meanwhile, the normal diet (ND) control group was given regular food alone throughout the study. The serum, liver, heart, and brain oxidative stress markers were assessed. Results: Kepel synbiotic supplementation consistently improved oxidative stress by decreasing malondialdehyde (MDA) levels and increasing superoxide dismutase (SOD) activity inhibition rate in serum, liver, heart, and brain in the HFD group compared to the ND group. This improvement effect occurred in a dose-dependent manner, increasing in higher kepel synbiotic doses. Conclusion: Kepel synbiotics showed a beneficial effect in improving oxidative stress in the serum, liver, heart, and brain of HFD-fed rats. Supplementation of kepel synbiotic can be considered a complementary therapeutic agent in improving oxidative stress, especially due to HFD consumption.

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