Background: Occupation plays a major role in the well-being of an individual and has an influence on oral health. Fishing is one such occupation that entails a lot of physical labour and encourages habits that lead to poor oral health. Therefore, it is critical to shed light on the oral health of this isolated population to improve their quality of life by various means. The aim of the study was to assess and compare the prevalence of dental caries, oral hygiene status and treatment needs of fisherman and non-fisherman population in South Goa, India. Materials and methods: Study design was cross-sectional in nature. After a pilot study, multi-stage random sampling technique was employed and 400 study participants were recruited. World Health Organization Oral Health Assessment Form (1997) and Oral Hygiene Index-Simplified (OHI-S) were used to record the study variables. Inter-examiner reliability assessed using Kappa statistics were found to be 90% and 88%, respectively. The data was analysed using descriptive analysis, Chi-square test, Mann-Whitney U test, Kruskal-Wallis test, and linear and logistic regression analysis. Results: Fishermen had significantly higher caries prevalence (82%) and poor oral hygiene (46%) than non--fishermen. Extraction (42.2%) and pulp care (23.6%) were the highest treatment need among fishermen. They were 2.08 times more prone to dental caries than non-fishermen. Fishermen who used a toothbrush were 4.5 times less susceptible to caries. The dependence of caries prevalence and OHI-S score on occupation, oral hygiene aid and age were 14% and 25.8%, respectively. Conclusions: Fishermen in South Goa had high caries prevalence, poor oral hygiene status and they required extensive dental treatment when compared to non-fishermen.
Aim To prepare and characterize a 6-gingerol-incorporated chitosan biopolymer for coating on thermoformed aligners and evaluate its scratch resistance and antimicrobial activity. Material and methods In this in vitro study, 6-gingerol extract was prepared, incorporated with chitosan biopolymer into a coating solution and characterized using nuclear magnetic resonance imaging spectroscopy (NMR). Twenty thermoformed aligner samples were exposed to UV radiation for surface activation, then coated with a crosslinking agent. These were divided into four groups of five. The control group consisted of samples dip-coated in a chitosan solution for 15 minutes. The three test groups consisted of samples dip coated in a gingerol-chitosan coating solution, with each group representing the following time periods of dip coating: five, 10, and 15 minutes. The crosslinking of the coating with the aligner material was confirmed by a Fourier transform infrared spectroscopy (FTIR) test. A scratch test was carried out to evaluate the wear resistance of the coating, and the antibacterial properties of the coating were tested using a Disc Diffusion test. Results The NMR analysis confirmed the presence of 6-gingerol in the extract. The coating of 6-Gingerol on aligners was confirmed by FTIR spectroscopy. The wear resistance of aligners coated for 5 minutes, 10 minutes, and 15 minutes was 1.8 ± 0.09 N, 2.3 ± 0.021 N, and 3.06 ± 0.17 N, respectively, and the difference was statistically significant (p<0.05). The aligner coated for 15 minutes exhibited the widest zone of inhibition of up to 2.38 ± 0.44 mm against Streptococcus mutans , and the difference was statistically significant (p<0.05). No antibacterial effect was found against E. Coli . Conclusion A novel coating material with 6-gingerol extract incorporated in chitosan biopolymer was prepared and characterized, followed by coating on thermoformed aligners. The coating showed antibacterial activity against Streptococcus mutans, and both the antimicrobial activity and wear resistance increased with coating duration.
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