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Thirty yellow inbred lines of normal maize were evaluated for thirteen parameters at the experimental field of Hajee Mohammad Danesh Science and Technology University during 2010-11 to study the genetic divergence using multivariate analysis. The thirty inbreds fell into six distinct clusters. The intra-cluster distances in all the six clusters were more or less low, indicating that the genotypes within the same cluster were closely related. The highest inter-cluster distance was observed between cluster I and cluster IV and the lowest between the cluster II and III. The cluster V and cluster IV contained the highest (9) and lowest (1) number of genotypes, respectively. Cluster VI showed the highest mean values for kernel yield and all the yield contributing traits except days to 50% tasseling and 50% silking. Cluster II had the lowest mean values for ear height and ear length. Days to maturity and ear diameter showed maximum contribution towards total divergence among different characters. Based on medium to high inter-cluster distances, six inbred lines viz. ML06, ML10, ML14, MK19, ML25 and ML26 were selected for hybrid program. Development of hybrids utilizing these genotypes has the chance to obtain higher heterosis with high performing crosses.

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Summary Hibiscus cannabinus var. HC-2 (2nϭ2xϭ36) and H. sabdariffa var. HS-24 (2nϭ4xϭ72) were cytogenetically and biochemically studied to elucidate their genomic homology. Both the species possessed only metacentric chromosomes. The range of chromosome length of these 2 species was 1.43-3.80 mm. Both the species showed a gradual decrease in chromosome length. Six CMA-positive bands were found in both the species. Two entirely CMA-positive banded chromosomes were found in both the species. Twenty six DAPI-positive bands were found in H. cannabinus var. HC-2. In H. sabdariffa var. HS-24, 14 DAPI-positive bands appeared. These bands were distributed in different location of respective chromosomes. Two chromosomes of H. cannabinus var. HC-2 and 8 chromosomes of H. sabdariffa var. HS-24 were entirely banded with DAPI. The entirely CMA-and DAPI-banded chromosomes were unique and could be used as marker. In H. cannabinus var. HC-2, a pair of satellites was found in orcein and CMA-staining. No satellite was found in DAPI-staining indicates its GC-rich nature. The activity of acid phosphatase and peroxidase was found similar in both the species. However, the 2 species showed different activities for esterase. The karyotypes (conventional and fluorescent) and electrophoresis pattern (acid phosphatase and peroxidase) indicates that either out of 2 different genomes in H. sabdariffa var. HS-24, 1 is very close to that of H. cannabinus var. HC-2 or H. sabdariffa var. HS-24 and H. cannabinus var. HC-2 may have derived their part of genomes from a common ancestor.