Multiple sclerosis (MS) is a demyelinating ailment in which the loss of myelin destructs conduction along the affected axons typically resulting in the conduction block. The motive of this protocol is to carry out the neuroprotective activity of Artemisia vulgaris (Mugwort) by induced demyelination of ethidium Bromide in wistar rats. The leaf methanolic extract was treated at 100mg/kg and 200mg/kg weight administered through oral route and continued for 28 days in demyelinated rats. Demyelination was affected by administering intracranial injection of toxin (ethidium Bromide) at the dose of 1µg/0.03ml of PBS per kg body weight. The potency of the extract was analysed in the terms of their behavioural study on the first, second and fourth week. The animals were immolate after 28 days and subjected to histopathological assessment. The unearthing from behavioural histopathological and biochemical studies evince that the methanol extract of Artemisia vulgaris have potential protective effect on the ethidium bromide induced demyelinated rats.

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Multiple sclerosis (MS) is a chronic disabling disease of the CNS that affects people during early adulthood. Despite several US FDA-approved medications, the treatment options in MS are limited. Many people with MS explore herbal products treatments to help control their MS and treat their symptoms. Surveys suggest that up to 70-80% of people with MS had tried plants products or bioactive compounds of plants for their MS. Patients with MS using herbal products potentially explore a new area for the research. The phyto therapies most frequently used include diet, omega-3 fatty acids and antioxidants. The most promising therapy for the anti-inflammatory and neuroprotective agents in both relapsing and progressive forms of MS were bioactive compounds of plant like flavonoids, vitamin-c, lipoic acid and vitamin D supplementation and others. In future polyphenols, terpenes, alkaloids, anthocyanidine glycosides, plant amines, volatile oils etc. would play a prominent role in the treatment of MS.

Clitoria ternatea, Cucurbita maxima, Artemisia vulgaris were the Indian native plants used in the treatment of different diseases. The methanolic extract of Clitoria ternatea, petroleum ether extract of Cucurbita maxima and methanolic extract of Artemisia vulgaris were investigated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. These extracts were assessed using N2a cell line (Neuroblastoma). Cisplatin was used as a positive control based on IC50 values. Artemisia vulgaris methanol extract have highest cytotoxicity (80.81ug/mL) and Cucurbita maxima seed extract have lowest cytotoxicity (57.655ug/mL). The comparison of IC50 of the extracts had shown the following toxicity: Cucurbita maxima petroleum ether extract <Clitoria ternatea methanolic extract <Artemisia vulgaris methanolic extract. The Cucurbita maxima showed least cytotoxicity against the tested cell lines. The results indicate that Cucurbita maxima seed extract have significant potential to be used as a natural anticancer agent in cultured N2a cell lines.