The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.

Currently, the point of care testing (POCT) is not fully developed for subtype-specific avian influenza virus detection. In this study, an H5N1 hemaglutinin 1 (HA1) epitope (P0: KPNDAINF) and three modified peptides (P1: KPNTAINF, P2: KPNGAINF, P3: KPNDAINDAINF) were evaluated as POCT elements for rapid detection of avian influenza virus. Based on modeling predictions by Autodock Vina, binding affinity varied depending on alteration of one amino acid in these peptides. The binding energy of P2 indicated its potential for a strong interaction with HA. Fluorescence-linked immunosorbent assay experimentally demonstrated the interaction between these peptides and virus. The four peptides interacted with HA1 of H5N3 with different binding affinities with P2 showing the strongest binding affinity. When P0 and P2 peptides were used in rapid fluorescent immunochromatographic test (FICT) as detection elements, the inter-assay coefficients of variation (CV) indicated that P2-linked FICT was more acceptable than the P0-linked FICT in the presence of human specimens. Antibody pair-linked FICT was influenced by clinical samples more than the P2-linked FICT assay, which showed a 4-fold improvement in the detection limit of H5N3 and maintained H5 subtype-specificity. Compared to the rapid diagnostic test (RDT) which is not specific for influenza subtypes, P2-linked FICT could increase virus detection. In conclusion, results of this study suggest that HA epitope-derived peptides can be used as alternatives to antibodies for a rapid fluorescent diagnostic assay to detect avian influenza virus.

Empirical studies exploring particular mechanisms that the country's institutional profile, such as regulatory and normative dimension are internalised into scientists' cognitive process of commercialising their research results, are rather sparse. This study aims to develop a conceptual framework in which integrated the lens of institutional theory and theory of planned behaviour to empirically test and discover the effects of regulatory and normative supports on scientists' intention to commercialise an invention. A survey was conducted at institutions and universities using stratified random sampling from 255 scientists during the period of 2 months from May to July 2020. A structural equation modelling was employed to estimate the paths and correlations. Results revealed that although regulatory support was not found to have the direct role in shaping intention to commercialise the invention, it had more influences and significances in the increase of attitude towards entrepreneurship, subjective norms, perceived behavioural control. Also, attitude towards entrepreneurship and perceived behavioural control were found to be full mediators in the linkage between regulatory support and intention to commercialise the invention, but subjective norms were not. Besides, normative support, although it was not related to subjective norms, it had direct influences on attitude toward invention commercialisation, perceived behaviour control, and intention to commercialise the invention. Thus, attitude toward invention commercialisation, perceived behaviour control was figured out to play the partially mediating role in the relationship between normative support and intention to commercialise invention among scientists. This research shows that new institutions that address younger generations and concentrate on enhancing entrepreneurial qualities are crucial to foster commercialisation in emerging economies. Also, by representing outstanding issues, our research encourages further debate and research activities. Implications for Central European audience: Even though the research results are related to academic entrepreneurship in Vietnam, the findings of this study can advocate that to promote academic entrepreneurship in emerging economies, enhancing both regulatory and normative supports are really necessary. In addition to theoretical contributions, this study also has several practical implications for researchers and policymakers in Central Europe.

This study aims to investigate the effects of internal barriers, including personal traits and cognitive conditions, and external barriers, including normative and regulative structures, on entrepreneurial intention among Vietnamese students. By collecting data from 437 students at high schools, universities and colleges in Vietnam, authors employ a quantitative method such as certain descriptive statistics, explorative analysis (EFA), KMO and Bartlett test, correlation coefficient analysis, and logistic regression to examine the relationship between entrepreneurial barriers with different issues including personal traits, cognitive condition, normative and regulative structures, and entrepreneurial intention. The research results show that all four independent variables; namely barriers related to personal traits, cognitive condition, normative and regulative structures had negative effects on entrepreneurial intention. Particularly, cognitive conditions are seen as the most influential barrier to entrepreneurial intention, followed by personal traits, regulative and normative structures.

Manuscript type: Research paper. Research aims: This study investigates the relationship between cash conversion cycle and profitability that exists in the agriculture and food industries in Thailand. It specifically aims to examine the influence of production cycle, cash collection cycle, and cash payment cycle on profitability. In addition, it also aims to measure the influence of control variables such as size and debt ratios have on profitability. Design/ Methodology/ Approach: This study analysed the data of 34 listed companies in agriculture and food industry in the Stock Exchange of Thailand from 2009 to 2013. Pearson's correlation and the regression analysis approach were used to examine the relationship between cash conversion cycle and profitability. Research findings: The results indicate that cash conversion cycle (CCC) has a significant inverse relationship with profitability in the agriculture and food companies in Thailand. Further, production cycle and debt ratio were found to have a significant negative relationship with return on assets (ROA) while payment cycle and size have a positive relationship with return on equity (ROE). No significant relationship was found between cash collection cycle and profitability. Theoretical contributions/ Originality: This study expands on the theoretical concepts of cash conversion cycle and its effects on profitability. Although studies have been done on manufacturing