Hydrophobic curcumin in temulawak extract and hydrophilic betacyanin in red dragon fruit extract are high-value bioactive compounds with extensive applications in functional food. In this study, these extracts were encapsulated in water-in-oil-in-water (w/o/w) nanoemulsions as a delivery system using a two-step high-energy emulsification method. PGPR and Span 20 were used as lipophilic emulsifiers for the primary w/o emulsion. The most stable w/o/w formulation with the least oil phase separation of 5% v/v consisted of w/o emulsion (15% w/w) and Tween 80 (1.5% w/w) as hydrophilic emulsifier. The formulation was characterized by a 189-nm mean droplet diameter, 0.16 polydispersity index, and –32 mV zeta potential. The freeze–thaw stability may be attributed to the combination of low w/o emulsion content and high Tween 80 concentration in the outer water phase of the w/o/w nanoemulsions used in this study. The IC50 values of the nanoemulsion and the red dragon fruit extract were similar. It means that the higher concentration of curcumin in the nanoemulsions and the lower IC50 value of temulawak extract ensured sufficient antioxidant activities of the w/o/w nanoemulsions.

<p>Minyak biji pala mengandung senyawa bioaktif yang memiliki aktivitas antimikroba. Senyawa bioaktif umumnya bersifat tidak stabil sehingga mudah mengalami penurunan aktivitas biologisnya. Salah satu cara untuk meningkatkan stabilitasnya adalah dengan menggunakan teknologi nano. Penelitian ini bertujuan untuk (1) mendapatkan formulasi nanoemulsi minyak biji pala dengan ukuran droplet partikel &lt;300 nm sebagai bahan antimikroba, (2) mendapatkan formulasi nanoemulsi yang memiliki aktivitas antimikroba terbaik terhadap mikroba E.coli, S.aureus dan S.cereviseae. Proses nanoemulsi minyak biji pala dilakukan dengan menggunakan alat High Pressure Homogenizer pada tekanan 20000 psi atau 137,931 KPa dengan 5 siklus. Pengamatan dilakukan terhadap 12 formulasi yang diperoleh dari kombinasi antara tiga konsentrasi minyak (5%, 10% dan 15%) dan dua jenis surfaktan (tween 20 dan tween 80) dengan tiga tingkat konsentrasi (10%, 15% dan 20% dari massa minyak biji pala). Pengujian aktivitas antikmikroba dilakukan dengan metode difusi sumur. Hasil penelitian menunjukkan bahwa formulasi nanoemulsi minyak biji pala yang diperoleh memiliki ukuran droplet partikel 104,80-161,15 nm.. Konsentrasi minyak biji pala berpengaruh secara signifikan terhadap zona penghambatan pertumbuhan mikroba. Nanoemulsi minyak biji pala dengan formulasi konsentrasi minyak biji pala 15% dan jenis surfaktan Tween 80 dengan konsentrasi 20% dari massa minyak (M15S20T80) memberikan efek penghambatan terbaik pada E.coli (11,25 mm), S.aureus (13,06 mm) dan S.cereviseae (11,4 mm).</p><p>Kata kunci :minyak biji pala, surfaktan, nanoemulsi, antimikroba, penghambatan</p><p>English Version Abstract</p><p>Nutmeg oil contained bioactive compounds which had antimicrobial activities. Generally, bioactive compounds have a limited stability, so that they undergo biological activity degradation easily. One of ways to increase its stability is using nanotechnology. This research was aimed (1) to obtain nutmeg oil nanoemulsion formulation which having particle size &lt;300 nm as antimicrobial agent, (2) to obtain nanoemulsion formulation which having the best antimicrobial activity toward E.coli, S.aureus and S.cereviseae. Processing of nanoemulsion was conducted using High Pressure Homogenizer at 20000 psi or 137,931 Kpa and 5 cycles. Observation was conducted to 12 nanoemulsion formulation which was gained from combination between concentration of nutmeg oil (5%, 10%, 15%) and types of surfactant (tween 20 and tween 80) with 3 level concentration (10%, 15% and 20% from nutmeg oil mass). Antimicrobial testing was conducted using agar well diffusion method. The result showed that formulation of nutmeg oil nanoemulsion having particle size 104,80-161,15 nm. Nutmeg oil concentration had significantly effect in inhibition zone of microbes growth. Formulation of nutmeg oil nanoemulsion with 15% nutmeg oil and surfactant tween 80 as much as 20% of nutmeg oil mass gave the best growth inhibition on E.coli (11,25 mm), S.aureus (13,06 mm) dan S.cereviseae (11,4 mm).</p><p>Keywords :nutmeg oil, nanoemulsion, antimicrobial, inhibition</p>

Clove oil has benefits for both food and pharmaceutical purposes. However its use is limited due to several properties such as susceptibility to oxidation, poor water solubility, react with other components, and toxic at high concentration. The limitation of its usage can be overcome through emulsion technology. Therefore, this study aims to obtain clove oil nanoemulsion through preparation using whey protein-maltodextrin conjugates. These conjugates act as an emulsifier and a first layer, meanwhile chitosan as a stabilizer and also a second layer. The formulation used different chitosan concentrations, at 0.2, 0.4, and 0.6%, then processed using High-Pressure Homogenizer (HPH) in 3 and 5 cycles. The result showed that the concentration of chitosan and the number of HPH cycles affected the clove oil emulsion performance. Furthermore, the formulation using 0.6% chitosan concentration and 5 cycles of HPH produced the best clove oil nanoemulsion in droplet size, polydispersity index, and zeta potential were 147.5, 0.214, and -52.4, respectively. The results of this study provide information on the potential of emulsion formulations using WPM conjugate and chitosan to produce clove oil nanoemulsions that can be utilized in food and pharmaceutical products.

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